乳腺癌患者血清长链非编码RNA mir4435-2HG表达水平与临床病理特征的相关性研究

Correlation between Expression Level of Serum Long Non-coding RNA mir4435-2HG and Clinicopathological Characteristics in Breast Cancer Patients

目的探究乳腺癌(breast cancer,BC)患者血清长链非编码RNA(long non-coding RNA,lnc RNA)mir4435-2HG表达水平与临床病理特征的相关性。方法选择2016年8月~2017年7月长安大学医院收治的52例BC患者为BC组,选择同期49例乳腺良性疾病患者为乳腺良性疾病组,另随机纳入同期45例体检健康的志愿者为健康对照组。采用实时荧光定量PCR(Quantitative real-time PCR,qRT-PCR)检测血清lnc RNA mir4435-2HG,通过比较各组血清中lnc RNA mir4435-2HG相对表达量的差异,分析lnc RNA mir4435-2HG与患者各临床病理特征的关系。lnc RNA mir4435-2HG的诊断价值分析采用ROC曲线。结果血清lnc RNA mir4435-2HG在BC组[2.04(1.41,3.96)],乳腺良性疾病组[1.21(1.00,1.39)]和健康对照组[0.99(0.84,1.15)]的相对表达量比较差异具有统计学意义(H=28.73,P=0.00),BC组血清lnc RNA mir4435-2HG的相对表达量高于乳腺良性疾病组(U=89.23,P=0.01)和健康对照组(U=91.46,P=0.02)。BC患者血清lnc RNA mir4435-2HG与年龄和肿瘤大小无显著相关性(U=115.41,155.02,均P>0.05),而与淋巴结转移、TNM分期、ER,PR和Her2有显著相关性(U=87.32,90.51,102.74,98.73,91.50,均P<0.05)。lnc RNA mir4435-2HG诊断BC的最佳截断值为0.69,该截断值对应的灵敏度和特异度分别为80.25%和70.91%。结论lnc RNA mir4435-2HG在BC血清中高表达,可作为BC的潜在生物学标志物。

Objective To explore the correlation between the expression level of long non-coding RNA(lnc RNA)miR4435-2HG in serum and clinicopathological characteristics in breast cancer(BC)patients.Methods Fifty-two BC patients admitted to Chang’an University Hospital from August 2016 to July 2017 were selected as the BC group,49 benign breast disease patients were selected as the benign breast disease group,and 45 healthy volunteers during the same period were randomly included as the healthy control group.Quantitative real-time PCR(qRT-PCR)was used to detect serum lnc RNA mir4435-2HG,and the relationship between lnc RNA mir4435-2HG and the clinicopathological characteristics of patients was analyzed by comparing the relative expression levels of lnc RNA mir4435-2HG in serum of each group.The diagnostic value of lnc RNA miR4435-2HG was analyzed using ROC curve.Results The relative expression level of serum lnc RNA mir4435-2HG in BC group[2.04(1.41,3.96)],benign breast disease group[1.21(1.00,1.39)]and healthy control group[0.99(0.84,1.15)]was statistically significant(H=28.73,P=0.00).The relative expression level of serum lnc RNA mir4435-2HG in BC group was higher than that in benign breast disease group(U=89.23,P=0.01)and healthy control group(U=91.46,P=0.02).Serum lnc RNA mir4435-2HG in BC patients was not significantly correlated with age and tumor size(U=115.41,155.02,all P>0.05),but was significantly correlated with lymph node metastasis,TNM stage,ER,PR and HER2(U=87.32,90.51,102.74,98.73,91.50,all P<0.05).The optimal truncation value of lnc RNA mir4435-2HG in the diagnosis of BC was 0.69,and the corresponding sensitivity and specificity were 80.25%and 70.91%,respectively.Conclusion lnc RNA mir4435-2HG was highly expressed in BC serum and could be used as a potential biomarker of BC.