多囊卵巢综合征患者血清Spexin水平表达及其与多项生理生化指标的相关性分析

Expression of Serum Spexin in Patients with Polycystic Ovary Syndrome and Its Correlation with Multiple Physiological and Biochemical Indexes

目的探究多囊卵巢综合征(polycystic ovarian syndrome,PCOS)患者血清Spexin水平表达及其与多项生理生化指标的相关性。方法选取西安高新医院2018年9月~2019年10月收治的60例PCOS患者为观察组,另选取同期体检健康者60例为对照组,采用全自动生化分析仪检测受试者的总胆固醇(total cholesterol,TC)和低密度脂蛋白胆固醇(low density lipoprotein-cholesterin,LDL-C),采用电化学发光免疫分析法(ECLIA)测定血清卵泡刺激素(follicle-stimulating hormone,FSH)和雌二醇(estradiol,E2)的水平,采用酶联免疫吸附试验(ELISA)测定血清Spexin水平。根据血清Spexin水平的不同将受试者又分为低表达组(Spexin≤2.8 ng/ml)和高表达组(Spexin>2.8 ng/ml)两组。比较两组的一般资料,Spexin高、低表达组多项生理生化指标并采用Spearman相关性检验分析其相关性。结果两组的生化指标比较差异无统计学意义(t=0.04~1.71,均P>0.05),观察组血清Spexin水平5.26±0.37 ng/ml显著高于对照组2.74±0.21 ng/ml,差异具有统计学意义(t=11.63,P<0.05)。Spexin高、低表达组的TC,LDL-C,FSH和E2的差异均无统计学意义(t=0.216~0.495,均P>0.05),Spexin高、低表达组的BMI水平差异具有统计学意义,并且Spexin高表达组的BMI显著高于Spexin低表达组(t=5.913,P<0.05)。Spearman相关性检验分析结果显示血清Spexin水平与BMI呈正相关关系(rs值为0.074,P<0.05)。结论Spexin在PCOS中高表达并且其受BMI的影响。

Objective To investigate the expression of serum Spexin in patients with polycystic ovarian syndrome(PCOS)and its association with multiple physiological and biochemical indexes.Methods A total of 60 PCOS patients in Xi’an Gaoxin Hospital from September 2018 to October 2019 were selected as the observation group,and 60 healthy subjects were selected as the control group during the same period.Applying automatic biochemical analyzer to test total cholesterol(TC)and low-density lipoprotein-cholesterol(LDL-C).Applying ECLIA to measure levels of follicle-stimulating hormone(FSH)and estradiol(E2).Applying ELISA to measure the level of serum Spexin.Subjects were further divided into two groups according to the serum Spexin level,namely the low-expression group(Spexin≤2.8 ng/ml)and the high-expression group(Spexin>2.8 ng/ml).The general data of the two groups,as well as multiple physiological and biochemical indexes of the groups with high and low Spexin expression were compared,and the correlation between them was explored by Spearman correlation analysis.Results There were no statistically significant differences between the two groups(t=0.04~1.71,all P>0.05).The serum Spexin level of the observation group(5.26±0.37 ng/ml)was higher than that of the control group(2.74±0.21 ng/ml),and the difference was statistically significant(t=11.63,P<0.05).There were no statistically significant differences in TC,LDL-C,FSH and E2 in the high and low Spexin expression groups(t=0.216~0.495,all P>0.05),while there was statistically significant differences in BMI level in the high and low Spexin expression groups,the BMI of the group with high Spexin expression was significantly higher than that of the group with low Spexin expression(t=5.913,P<0.05).Spearman correlation analysis showed that serum Spexin level was positively correlated with BMI(rs value was 0.074,P<0.05).Conclusion Spexin was highly expressed in PCOS and was affected by BMI.