党参多糖对番泻叶所致脾虚模型小鼠的补脾作用研究

Study on the Tonifying Spleen Effect of Codonopsis pilosula Polysaccharides on Sennae Folium-induced Spleen-deficiency Model Mice

目的:研究党参多糖对脾虚模型小鼠的补脾作用。方法:将60只雄性ICR小鼠随机分为空白组,模型组,党参多糖高、中、低剂量组[1.6、0.8、0.4 g/(kg·d)]和四君子汤组[30 g/(kg·d),以生药总量计],每组10只。除空白组外,其余各组小鼠均灌胃番泻叶溶液(0.4 g/d)构建脾虚模型。造模后,各给药组小鼠灌胃相应药液,空白组和模型组小鼠灌胃20 mL/kg生理盐水;每天给药1次,连续6周。给药结束后,测定小鼠体质量并进行一般行为学观察,记录其全血中红细胞计数,测定其血清中D-木糖、胃泌素(GAS)、胃动素(MTL)、生长抑素(SS)、血管活性肠肽(VIP)、淀粉酶(AMS)、免疫球蛋白(IgG和IgM)、脂多糖(LPS)含量,并测定其结肠组织中密封蛋白(Claudin)、闭合蛋白(Occludin)mRNA及其蛋白表达水平。结果:与空白组比较,模型组小鼠体质量显著减轻(P<0.05),出现了背毛稀疏、无光泽等脾虚症状;全血中红细胞计数和血清中D-木糖、SS、VIP、AMS、IgG、IgM含量以及结肠组织中Claudin、Occludin mRNA及其蛋白的表达水平均显著降低(P<0.05或P<0.01),血清中GAS、MTL、LPS含量均显著升高(P<0.01)。与模型组比较,党参多糖高剂量组小鼠体质量显著增加(P<0.05),脾虚症状显著改善;除党参多糖低剂量组小鼠血清中D-木糖、IgM含量和结肠组织中Claudin、Occludin mRNA及其蛋白表达水平差异无统计学意义外(P>0.05),其余各组小鼠上述指标均显著改善(P<0.05或P<0.01)。结论:党参多糖对番泻叶所致的脾虚模型小鼠具有改善作用,其作用机制可能与调节其胃肠激素分泌、增强免疫以及保护肠黏膜屏障有关。

OBJECTIVE:To study the tonifying spleen effect of Codonopsis pilosula polysaccharides(CPP)on spleen-deficiency model mice.METHODS:Totally 60 ICR male mice were randomized into blank group,model group,CPP high-dose,medium-dose and low-dose groups[1.6,0.8,0.4 g/(kg·d)],Sijunzi tang(SJZT)group[30 g/(kg·d),by crude drug],with 10 mice in each group.Except for blank group,other groups were given Sennae Folium solution intragastrically(0.4 g/d)to establish spleen-deficiency model.After modeling,administration groups were given relevant drug intragastrically,and blank group and model group were given 20 mL/kg normal saline intragastrically,once a day,for consecutive 6 weeks.After last administration,body weight of mice in each group was measured and their general behavioral characteristics were observed.The red blood cell count in whole blood were recorded,and the contents of D-xylose,gastrin(GAS),motilin(MTL),somatostatin(SS),vasoactive intestinal peptide(VIP),amylase(AMS),immunoglobulin(IgG and IgM)and lipopolysaccharide(LPS)in serum were determined;mRNA and protein expressions of Claudin and Occludin in colon tissues of mice were also detected.RESULTS:Compared with blank group,body weight of mice in model group was significantly decreased(P<0.05),and the spleen deficiency symptoms such as sparse back hair and no luster appeared;the red blood cell count in whole blood,serum contents of D-xylose,SS,VIP,AMS,IgG and IgM,mRNA and protein expressions of Claudin and Occludin were significantly decreased(P<0.05 or P<0.01);serum contents of GAS,MTL and LPS were significantly increased(P<0.01).Compared with model group,the body weight of mice were increased significantly in CPP high-dose group(P<0.05),and spleen-deficiency symptom was improved significantly.Except for the serum contents of D-xylose and IgM,the protein expressions of Claudin and Occludin in colon tissue had no statistical significance in CPP low-dose group(P>0.05),above indexes of other groups were improved significantly(P<0.05 or P<0.01).CONCLUSION:CPP can improve spleen-deficiency model mice induced by Sennae Folium,the mechanism of which may be associated with regulating gastrointestinal hormone secretion,enhancing immunity and protecting intestinal mucosal barrier.