抑瘤素M、Id-1、Foxp3在胃癌组织中的表达及与病理特征的相关性

Expression of tumor suppressor M,Id⁃1 and Foxp3 in gastric cancer tissues and their correlation with pathological characteristics

目的探究抑瘤素M(OSM)、细胞分化抑制因子-1(Id-1)、Foxp3在胃癌组织中的表达及与病理特征相关性。方法选取2019年6月-2020年6月于我院进行手术治疗的胃癌患者72例,取所有患者手术切除的癌组织及癌旁组织标本进行免疫组化染色,Western blot法检测抑瘤素M、Id-1、Foxp3相对表达量,对胃癌患者癌组织中抑瘤素M、Id-1、Foxp3表达相关性进行分析。结果与癌旁组织相比,胃癌组织中抑瘤素M、Id-1相对表达量较高(P<0.05);与癌旁组织相比,胃癌组织中Foxp3相对表达量较低(P<0.05)。与无淋巴结转移患者相比,有淋巴结转移胃癌患者抑瘤素M、Id-1相对表达量较高,Foxp3相对表达量较低(P<0.05);与浆膜内浸润患者相比,浆膜外浸润胃癌患者抑瘤素M、Id-1相对表达量较高,Foxp3相对表达量较低(P<0.05);与Ⅰ、Ⅱ期患者相比,Ⅲ、Ⅳ期胃癌患者抑瘤素M、Id-1相对表达量较高,Foxp3相对表达量较低(P<0.05);与高分化患者相比,中分化胃癌患者抑瘤素M、Id-1相对表达量较高,Foxp3相对表达量较低;与中分化患者相比,低分化胃癌患者抑瘤素M、Id-1相对表达量较高,Foxp3相对表达量较低(P<0.05)。胃癌组织中抑瘤素M、Id-1表达呈正相关(r=0.310,P=0.008);胃癌组织中抑瘤素M、Foxp3表达呈负相关(r=-0.422,P=0.001);胃癌组织中Id-1、Foxp3表达呈负相关(r=-0.308,P=0.009)。结论抑瘤素M、Id-1、Foxp3在胃癌中呈现异常表达,且抑瘤素M、Id-1、Foxp3相对表达量变化与胃癌患者淋巴结转移情况、浸润程度、TNM分期、分化程度相关,三者表达之间具有一定相关性,共同参与胃癌发展演进过程。

Objective To investigate the expression of oncostatin M(OSM),inhibitor of cell Differentia⁃tion⁃1(Id⁃1)and Foxp3 in gastric cancer tissues and their correlation with pathological feature.Methods Seventy⁃two patients with gastric cancer who underwent surgical treatment in our hospital from June 2019 to June 2020 were selected.The resected cancer tissues and adjacent tissues of all patients were taken for immunohistochemical stain⁃ing,and the relative expression levels of inhibin M,Id⁃1 and Foxp3 were detected by Western blot.The correlation between the expression of inhibin M,Id⁃1 and Foxp3 in the cancer tissues of gastric cancer patients was analyzed.Results Compared with that in the adjacent tissues,the relative expression of tumor suppressor M and Id⁃1 was higher,but that of Foxp3 was lower in gastric cancer tissues(P<0.05).Compared with patients without lymph node metastasis,patients with gastric cancer with lymph node metastasis had higher relative expression of inhibin M and Id⁃1 but lower relative expression of Foxp3(P<0.05).Compared with patients with intraserous invasion,patients with extraserous invasion of gastric cancer had higher relative expression of inhibin M and Id⁃1 but lower relative expression of Foxp3(P<0.05).Compared with patients with stageⅠandⅡ,patients with stageⅢandⅣgastric cancer had lower relative expression of Foxp3(P<0.05).The relative expression of inhibin M and Id⁃1 was higher and the relative expression of Foxp3 was lower(P<0.05)in patients with moderately differentiated gastric cancer when compared with patients with moderately differentiated gastric cancer.The relative expression of inhibin M and Id⁃1 was higher and the relative expression of Foxp3 was lower in patients with poorly differentiated gastric cancer when compared with patients with moderately differentiated gastric cancer(P<0.05).The expres⁃sion of inhibin M and Id⁃1 was positivelycorrelated(r=0.310,P=0.008);but the expression of inhibin M and Foxp3(r=-0.422,P=0.001)and that of Id⁃1 and Foxp3 was negatively correlated(r=-0.308,P=0.009).Conclusion The expression of inhibin M,Id⁃1 and Foxp3 is abnormal in gastric cancer,and the relative expres⁃sion changes of inhibin M,Id⁃1 and Foxp3 are correlated with lymph node metastasis,invasion degree,TNM stage and differentiation degree in gastric cancer patients,and the expression of M,Id⁃1 and Foxp3 is correlated to some extent,which jointly participate in the development and evolution of gastric cancer.