血清血小板反应蛋白2在结直肠癌早期诊断中的应用价值研究

The Application Value of Serum THBS-2 in Early Diagnosis of Colorectal Cancer

目的:探讨血清血小板反应蛋白2 (THBS-2)检测在结直肠癌早期诊断中的临床应用价值。方法:应用ELISA法检测23例结直肠癌患者及34例健康对照者血清中THBS-2的水平,通过ROC曲线来确定血清THBS-2检测诊断结直肠癌的灵敏度和特异度,并通过计算约登指数(Youden index)来确定THBS-2的cutoff值;同时与临床常用标志物CEA进行对比分析。结果:健康对照组和结直肠癌患者组血清中THBS-2的平均水平分别为(37.66±12.91) ng/ml和(6.59±18.58)ng/ml(P<0.001);AUC为0.934,最佳cutoff值17.495 ng/ml,灵敏度87%,特异度100%;23例结直肠癌患者中血清THBS-2检测出阳性患者20例,CEA检测出阳性患者11例(P<0.001)。结论:与健康对照组相比,结直肠癌患者血清中THBS-2水平明显降低;早期诊断结直肠癌患者的灵敏度和特异度都很高,阳性检出率比CEA高,对结直肠癌的早期诊断具有非常重要的临床应用价值。

Objective:To develop the application value of serum THBS-2 in early diagnosis of colorectal cancer.Methods:The ELISA method was used to detect the level of THBS-2 in the serum of 23 patients with colorectal cancer and 34 healthy controls.The ROC curve was used to determine the sensitivity and specificity of serum THBS-2 in the diagnosis of colorectal cancer,and the Y ouden index was calculated to determine the cutoff value of THBS-2,and with the clinically commonly marker CEA was used to compare and analyze.Results:The average level of THBS-2 in the serum of the healthy control group and the colorectal cancer patient group were 37.66±12.91 ng/ml and 6.59±18.58 ng/ml(P<0.001).AUC was 0.934,and the best cutoff value was 17.495 ng/ml.The sensitivity was 87%and the specificity was 100%.Among 23 patients with colorectal cancer,20 were positive for serum THBS-2 and 11 were positive for CEA(P<0.001).Conclusion:The serum THBS-2 levels of colorectal cancer patients were significantly lower than that of healthy controls.Serum THBS-2 has a higher sensitivity,specificity and positive detection rate compared with serum CEA.Serum THBS-2 show very important clinical application value in the early diagnosis of colorectal cancer.