基于氮掺杂碳量子点荧光探针检测四环素

Fluorescence detection of tetracycline based on nitrogen doped carbon quantum dots

本文以淀粉为碳源,以L-酪氨酸为氮供体,通过一步水热法制备了氮掺杂碳量子点(N-CQDs)。研究表明,N-CQDs表面富含氨基、羧基、羟基等官能团,说明具有很好的水溶性。还发现在pH=6.00的KH2PO4-NaOH缓冲溶液中,四环素对N-CQDs有强荧光猝灭作用,且四环素浓度在1.6~16μmol·L^(-1)范围和16~100μmol·L^(-1)范围内与N-CQDs的荧光猝灭的强度呈现较好的线性关系,检出限为0.45μmol·L^(-1),依此构建了N-CQDs荧光探针测定四环素的方法。该方法用于四环素片含量的测定,回收率为98.4%~102%。

Starch carbon quantum dots(N-CQDs)doped with L-Tyrosine nitrogen were prepared by one-step hydrothermal method.The results showed that N-CQDs were rich in functional groups such as amino,carboxyl and hydroxyl groups,indicating that N-CQDs had good water solubility.It was also found that tetracycline had strong fluorescence quenching effect on N-CQDs in the pH=6.00 KH2PO4-NaOH buffer solution,and the concentration of tetracycline in the range of 1.6~16μmol·L^(-1)and 16~100μmol·L^(-1) showed a good linear relationship with the fluorescence quenching intensity of N-CQDs,and the detection limit was 0.45μmol·L^(-1).The method was applied to the determination of tetracycline,and the recovery was 98.4%~102%.