NU7026对人肝癌细胞HepG2生物学行为的影响

Effect of NU7026 on the biological behavior of human hepatocarcinoma cell HepG2

目的探讨NU7026对HepG2细胞的影响,为治疗肝癌提供理论基础。方法用NU7026作用HepG2细胞,将实验分为空白组、对照组和NU7026组。CCK8实验检测NU7026对肝癌HepG2细胞存活率的影响。生长曲线实验和克隆实验检测HepG2细胞增殖能力,划痕实验检测HepG2细胞迁移能力,流式细胞术检测HepG2细胞凋亡和周期。结果与0μmol/L组和DMSO组比较,15μmol/L和20μmol/L NU7026作用于HepG2细胞,细胞存活率明显下降(P<0.05);与DMSO组相比,10μmol/L NU7026作用于HepG2细胞24、48、72 h后,细胞存活率下降(P<0.05);10μmol/L NU7026作用HepG2细胞,细胞增殖能力和迁移能力下降(P<0.05),细胞凋亡率增加(P<0.05),细胞周期无明显改变。结论NU7026能抑制HepG2细胞增殖能力和迁移能力可能与促进细胞凋亡有关。

Aim To explore the effect of NU7026 on HepG2 cells,and provide a theoretical basis for the treatment of liver cancer.Methods HepG2 cells were treated with NU7026,and the experiment was divided into the blank group,the control group and the NU7026 group.The CCK8 assay was used to detect the effect of NU7026 on the survival rate of HepG2 cells.The growth curve assay and clone formation assay were used to detect the proliferation ability of HepG2 cells,and the scratch assay was used to detect the migration ability of HepG2 cells.Finally the cellular apoptosis and cell cycle were tested by flow cytometry.Results Compared with the 0μmol/L group and the DMSO group,after 15μmol/L and 20μmol/L NU7026 treated on HepG2 cells,the cell survival rate was significantly decreased(P<0.05);Compared with the DMSO group,after treating HepG2 cells with 10μmol/L NU7026 for 24,48,and 72 hours,the cell survival rate was significantly decreased(P<0.05);After 10μmol/L NU7026 treated HepG2 cells,the cell proliferation and migration ability decreased(P<0.05),the apoptosis rate was significantly increased(P<0.05),but the cell cycle did not change significantly.Conclusion NU7026 can inhibit the proliferation and migration of HepG2 cells,which may be related to the promotion of cell apoptosis.