不同施肥处理对蔬菜土壤中抗生素抗性基因多样性与丰度的影响

Effects of different fertilization treatments on the diversity and abundance of antibiotic resistance genes in vegetable soil

为探究不同施肥方式对土壤抗生素抗性基因(ARGs)分布特征的影响,选取了长期采用4种施肥处理(不施肥、单施化肥、单施有机肥、常规施肥)的蔬菜土壤样品,应用高通量荧光定量聚合酶链式反应方法测定了土壤ARGs的种类与丰度。结果表明:不同施肥处理土壤分别检测到12~59种ARGs亚型,有10种为核心ARGs;各处理土壤中共有且丰度较高的ARGs亚型为czcA(多重耐药类);常规施肥土壤中ARGs总相对丰度显著高于其他3种土壤(P<0.05);重金属Cu、Zn、Cd,营养因子TN、有机质、速效钾、有效磷与ARGs均呈显著正相关(P<0.05)。

In order to explore the influence of different fertilization treatments on the distribution characteristics of soil antibiotic resistance genes(ARGs),vegetable soil samples that have been long-term used 4 fertilization treatments(no fertilization,single application of chemical fertilizer,single application of organic fertilizer,and conventional fertilization)were selected.The high-throughput fluorescent quantitative polymerase chain reaction method was used to determine the type and abundance of soil ARGs.The results showed that a total of 12-59 ARGs subtypes were detected,of which 10 were core ARGs;the ARGs common and relatively abundant in each treatment soil was czcA(multi-drug resistance);the total relative abundance of ARGs in conventional fertilized soils was significantly higher than that of the other three soils(P<0.05);heavy metals Cu,Zn,Cd,nutrient factors TN,organic matter,available potassium,and available phosphorus were all significantly positively correlated with ARGs(P<0.05).