子宫内膜异位症中箈络细胞的识别及形态特征(英文)

Identification and morphological features of telocytes in endometriosis

目的:研究箈络细胞(telocytes)在人卵巢子宫内膜异位症组织中的分布和形态结构特征。方法:取卵巢囊肿患者组织标本,通过苏木精-伊红染色,诊断为卵巢子宫内膜异位症。同时,在透射电子显微镜下对该组织进行观察。结果:通过观察苏木精-伊红染色后的卵巢囊肿病灶组织,发现存在异位子宫内膜,进而确诊为卵巢子宫内膜异位症。透射电子显微术结果显示卵巢子宫内膜异位症中的确存在箈络细胞。箈络细胞胞体小,向外延伸极其细长的念珠状突起,突起部分别由膨大的粗段与细段交替组成;粗段包含许多细胞器,如内质网和线粒体;且明显可见自噬小体和脂滴。此外,箈络细胞通过突起直接接触或分泌囊泡间接联系而形成网络结构。结论:我们通过透射电子显微术证实子宫内膜异位症病灶中确实存在箈络细胞,并描述了其形态学特征。本研究为卵巢子宫内膜异位症提供了新的细胞成分,加深了对卵巢子宫内膜异位症病理方面的认识。

AIM:To investigate the distribution and morphological features of telocytes(TCs)in the tissues of patients with ovarian endometriosis(EMs).METHODS:The tissue specimens from patients with ovarian cyst were har-vested,and diagnosed as EMs by hematoxylin-eosin(HE)staining.Simultaneously,ultrathin sections of the tissues were observed under transmission electron microscope(TEM).RESULTS:The ectopic endometrium was observed in the fo-cus of the ovarian cyst by HE staining,so that EMs was diagnosed.The results showed that TCs were indeed present in ovarian EMs under TEM.TCs with small cell bodies extended specific long,thin and moniliform telopodes(Tps)which had thick and thin parts(namely podoms and podomers,respectively).Moreover,podoms contained many organelles,such as endoplasmic reticulum and mitochondria.It was found that TCs in EMs had significant autophagosomes and lipid droplet.Furthermore,TCs formed a network through direct contact via long Tps and indirect contact via vesicles.CONCLUSION:The TCs exist in ovarian EMs and their morphological features were demonstrated by TEM.This study pro-vides an original structural and functional perspective for the cellular components of ovarian EMs and improves our compre-hesion of the pathological aspects of EMs.