利用小RNA测序技术鉴定吉林省甘薯病毒

Identification of viruses in sweet potato samples collected from Jilin province by small RNA sequencing

在吉林省7个主要甘薯种植区共采集85份甘薯叶片样品,利用小RNA深度测序技术对混合样品进行检测,经RT-PCR和测序验证,鉴定出样品中存在10种病毒,包括6种RNA病毒和4种DNA病毒。分别是马铃薯Y病毒科马铃薯Y病毒属的甘薯羽状斑驳病毒Sweet potato feathery mottle virus(SPFMV)、甘薯潜隐病毒Sweet potato latent virus(SPLV)、甘薯G病毒Sweet potato virus G(SPVG)、甘薯C病毒Sweet potato virus C(SPVC)、甘薯2号病毒Sweet potato virus 2(SPV2);长线形病毒科毛形病毒属的甘薯褪绿矮化病毒Sweet potato chlorotic stunt virus(SPCSV);双生病毒科菜豆金色花叶病毒属的甘薯曲叶病毒Sweet potato leaf curl virus(SPLCV);玉米线条病毒属的甘薯无症状1号病毒Sweet potato symptomless virus 1(SPSMV1);花椰菜花叶病毒科杆状DNA病毒属的甘薯杆状DNA病毒B Sweet potato badnavirus B(SPBV-B)和甘薯隐症病毒Sweet potato pakakuy virus(SPPV)。

85 samples of sweet potato leaf were collected from seven main sweet potato areas in Jilin province.Ten species of sweet potato virus were identified,including six RNA viruses and four DNA viruses,by using small RNA deep sequencing,RT-PCR and sequencing.The 10 viruses were Sweet potato feathery mottle virus(SPFMV),Sweet potato latent virus(SPLV),Sweet potato virus G(SPVG),Sweet potato virus C(SPVC)and Sweet potato virus 2(SPV2)in genus Potyvirus,family Potyviridae;Sweet potato chlorotic stunt virus(SPCSV)in genus Crinivirus,family Closteroviridae;Sweet potato leaf curl virus(SPLCV)in genus Begomovirus,family Geminiviridae;Sweet potato symptomless virus 1(SPSMV1)in genus Mastrevirus,family Geminiviridae;Sweet potato badnavirus B(SPBV-B)and Sweet potato pakakuy virus(SPPV)in genus Badnavirus,family Caulimoviridae.