水旱两种栽培模式下海南山栏稻对白叶枯病抗性鉴定与评价

Resistance evaluation and molecular identification of Hainan Shanlan upland rice to bacterial blight under flooding and drying cultivation conditions

为研究山栏稻对白叶枯病抗性,本试验以17个山栏稻品种为材料,在大田与温室大棚中同时种植,于大田中观察山栏稻在水、旱两种栽培模式下对海口当地白叶枯病菌的自然抗性并测产;在温室中检测水、旱两种栽培模式下山栏稻对菲律宾白叶枯病菌小种PXO99(P6)、PX145(P7)的抗性;并以基因功能性标记检测其抗性基因,分析各指标与抗病性的关系。结果表明,大田环境下17个供试品种中有12个品种在水作栽培模式下感病指数高于旱作,有10个品种水作栽培模式下产量比旱作高。温室中用P6接种山栏稻,水作模式下仅有1个品种表现抗病,8个品种感病;旱作模式下2个品种表现抗病,3个品种感病。接种P7,水作时表现抗病和感病的品种数量分别为7个和2个;旱作时表现抗病的品种高达9个,仅1个品种感病。供试品种中有16个品种含有Xa1抗病基因,4个品种含有Xa27抗病基因,所有品种均不含xa13、Xa21抗病基因。因此认为,山栏稻对海口当地白叶枯病菌小种以及菲律宾白叶枯病菌小种都有一定抗性,且抗性受栽培条件的影响,山栏稻旱作时抗性强于水作。

In order to investigate the resistance of Shanlan upland rice to bacterial blight(BB),17 varieties of Shanlan upland rice were used as materials and planted in the field and greenhouse at the same time in this study.In the field,the resistance of Shanlan upland rice to Haikou local races of BB was observed and the yield was measured under flooding and drying cultivation conditions.In the greenhouse,the resistance to two Philippine races of BB,PXO99(P6)and PX145(P7)was evaluated under flooding and drying cultivation conditions and the functional marker method was used to detect its resistance genes and relationship between various factors and resistance.The results showed that the disease indices of 12 varieties from 17 varieties under flooding cultivation were higher than that under drying cultivation in the field.In addition,there were 10 varieties with higher yield under flooding cultivation than drying cultivation.After inoculated with P6 in the greenhouse,only one variety showed resistance and eight varieties were susceptible under flooding cultivation,while two varieties were resistant and three varieties showed susceptibility under drying cultivation.After inoculated with P7,seven varieties showed resistance and two were susceptible under flooding cultivation,and nine varieties showed resistance and only one was susceptible under drying cultivation.According to the functional marker detection,16 varieties contained Xa1 or its homologous gene and four varieties contained Xa27 gene.However,all varieties did not contain resistance genes xa13 and Xa21.Therefore,it is believed that Shanlan upland rice had certain resistance to the bacterial blight races of Filipino and Haikou.The resistance was affected by different cultivation conditions and the resistance was stronger under drying cultivation than flooding cultivation.