MeJA诱导OsPR1A的表达水稻对白叶枯病的抗性研究

Research of MeJA-induced OsPR1A expression enhanceing resistance of rice to bacterial blight

本研究采用免疫印迹技术(Western Blot, WB)在蛋白质水平上研究植物激素,尤其茉莉酸对OsPR1A表达的影响,增强对OsPR1A生物学功能的了解,并进一步探究茉莉酸对水稻白叶枯病的抗性机制。结果发现,在水稻离体叶片中OsPR1A受到外源茉莉酸甲酯(Methyl Jasmonate, MeJA)的强烈诱导;在水稻幼苗中,MeJA处理后OsPR1A在根中表达量增加。MeJA处理感病型水稻TP309幼苗后,与对照相比病斑长度缩短约1cm,OsPR1A于接菌后第6天被提前诱导表达,病斑生长受到抑制。MeJA处理水稻OsPR1a-RNAi植株,抗病表型明显,说明MeJA可以减轻转基因水稻OsPR1a-RNAi对白叶枯病菌(Xanthomonas oryzae pv. oryzae,Xoo)的敏感性。

In this research, Western Blot(WB) was used to study the effect of plant hormones, especially jasmonic acid, on the expression of OsPR1 A at protein level, so as to enhance the understanding of the biological function of OsPR1 A and further explore the resistance mechanism of jasmonic acid to rice bacterial blight. The results showed that OsPR1 A was strongly induced by exogenous Methyl jasmonate(MeJA) in rice detached leaves. In rice seedlings, the expression of OsPR1 A in roots increased after MeJA treatment. After the spraying of MeJA on the seedlings of susceptible rice TP309, the length of lesions was reduced by about 1 cm compared with the control. The expression of OsPR1 A was induced in advance at 6 days post-inoculation(dpi), and the growth of the lesion was inhibited. The resistance phenotype of rice OsPR1 a-RNAi plants treated with MeJA was obvious, which indicated that MeJA could reduce the sensitivity of transgenic rice OsPR1 a-RNAi plants to bacterial blight(Xanthomonas oryzae pv. oryzae, Xoo).