UGRP1与甲状腺结节性质、功能及BRAF基因的相关性

Correlation between UGRP1 and the properties, functions and BRAF gene expression of thyroid nodules

目的探讨甲状腺结节子宫球蛋白相关蛋白1(UGRP1)的表达与甲状腺结节性质、功能及BRAFV600E基因突变的关系。方法 153例甲状腺组织取自131例以甲状腺结节为主诉的患者,根据细针穿刺细胞学检查(US-FNAC)结果分为甲状腺结节组(47例)、桥本甲状腺炎组(38例)、甲状腺癌组(46例),正常甲状腺组(22例)取自良性结节旁正常甲状腺组织。通过免疫组化方法分析甲状腺细胞UGRP1的表达,分析4组患者UGRP1表达情况,UGRP1阳性组和阴性组甲状腺功能、超声特征以及与BRAFV600E基因突变的关系。结果桥本甲状腺炎、甲状腺结节和甲状腺癌组UGRP1阳性表达率分别为71.1%、4.3%和2.2%,正常甲状腺组织中UGRP1表达阴性,差异有统计学意义(P<0.05)。TT3、TT4、TSH在UGRP1阳性组和阴性组差异无统计学意义。UGRP1表达与BRAFV600E基因突变之间存在相关性(P=0.023,C=0.414)。结论甲状腺结节中,桥本甲状腺炎UGRP1阳性高表达,UGRP1可能作为区分桥本甲状腺炎与正常甲状腺和甲状腺癌的参考指标之一。

Objective To investigate the relationship between the expression of uteroglobin-related protein 1(UGRP1) in thyroid cells and the properties, functions and the mutation of BRAFV600E gene in patients of thyroid nodules.Methods According to the results of cytological examination, 153 cases of aspirated thyroid tissues from 131 patients with thyroid nodules as the chief complaints undergone thyroid fine needle aspiration cytology(FNAC) examinations, were divided into thyroid nodules group(47 cases), Hashimoto′s thyroiditis group(38 cases) and thyroid carcinoma group(46 cases). Thyroid tissues adjacent to benign nodules were classified as normal thyroid group( 22 cases). The expressions of UGRP1 in thyroid cells obtained by FNAC were detected by immunohistochemistry,and the expressions of UGRP1 in the four groups were analyzed,as well as the thyroid functions,ultrasonic characteristics,the relationship between UGRP1 expression and BRAFV600Egene mutation in the positive and negative UGRP1 expression groups. Results The positive UGRP1 expression rates in Hashimoto’ s thyroiditis group,thyroid nodules group and thyroid carcinoma group were 71. 1%,4. 3% and 2. 2% respectively. UGRP1 was negatively expressed in normal thyroid group. There was significant difference between the four groups( P <0. 05). There was no significant difference in TT3,TT4 and TSH between the positive and negative UGRP1 expression groups. There was a correlation between UGRP1 expression and BRAFV600E gene mutation( P = 0. 023,C =0. 414). Conclusion In thyroid nodules,Hashimoto’s thyroiditis was highly positive expression for UGRP1. The positive expression of UGRP1 may be one of the reference indexes to distinguish Hashimoto’s thyroiditis from normal thyroid and thyroid carcinoma.