不同浓度锶与淫羊藿苷联合应用对MC3T3-E1细胞增殖及骨向分化的影响

Effects of strontium combined with icariin on proliferation and osteogenic differentiation of MC3T3-E1 cells under different concentrations

通过体外培养前成骨细胞MC3T3-E1细胞,加入不同浓度锶(Sr)与淫羊藿苷(ICA)处理,在第1、4、7和11天时,通过荧光显微镜观察细胞形态并对细胞计数,通过CCK-8法检测不同浓度Sr与ICA对前成骨细胞MC3T3-E1细胞增殖活性的影响。通过碱性磷酸酶(ALP)活性检测(第4和7天)及茜素红S染色(第21天),分析不同浓度Sr与ICA对MC3T3-E1细胞骨向分化的影响。结果显示,与其他组比较,S1I2组(80 mg/L Sr+7×10-2 mg/L ICA)在荧光显微镜下细胞数量最多,细胞增殖活性最强,并显示出最高的ALP活性和更多地红染的矿化结节。说明适宜浓度的Sr与ICA联合应用可有效促进前成骨细胞MC3T3-E1细胞增殖及骨向分化。

MC3 T3-E1 cells were cultured and treated with different concentrations of strontium and icariin. On the 1 st, 4 th, 7 th and 11 th day, cell morphology was observed and cell number was calculated under fluorescent microscope. CCK-8 method was also applied to analyze the effect of strontium and icariin on proliferation of MC3 T3-E1 cells. Alkaline phosphatase(ALP) activity detection(on 4 th and 7 th day) and Alizarin red staining(on 21 th day) were performed to detect the effect of strontium and icariin on osteogenic differentiation of MC3 T3-E1 cells. The results showed that compared with other groups, group S1 I2(80 mg/L Sr+7×10-2 mg/L ICA) had more MC3 T3-E1 cells and exhibited higher cell proliferation activity. Furthermore, ALP activity was the highest in S1 I2 group and more red-stained mineralization nodules were observed in group S1 I2. These suggest that the combination of strontium and icariin can promote proliferation and osteogenic differentiation of MC3 T3-E1 cells under adequate concentrations.