肠道菌群多样性与IgA肾病关联性研究

Associations between intestinal flora diversity and IgA nephropathy

目的肠道菌群通过影响肠道黏膜来参与人体免疫调节,菌群分布及菌群多样性的改变会使机体的免疫反应发生变化,本研究通过对比IgA肾病患者与正常人群肠道菌群的分布特点与差异,来初步阐释IgA肾病患者的肠道菌群分布特点。方法本研究收集了2018年10月至2019年10月之间在新疆维尔自治区人民医院行肾穿刺活检并诊断为IgA肾病患者的粪便标本,为IgA组,同时收取健康人群粪便标本,作为正常对照组。每组样本20例,分别送检行生物多样性分析,分析内容包括OTU分析、物种分类分析、Alpha多样性分析、Beta多样性分析等。结果在IgA组有7种不同的OTU区别于正常对照人群,进行组间LEfSe分析时提示肠杆菌目、肠杆菌科在导致样品物种差异方面的影响较大。从物种组成上看,健康对照组的物种组成较IgA组的更为丰富,同时物种均匀程度也较IgAN患者高。结论IgA肾病患者的肠道菌群多样性减少,同时优势菌群发生改变。本研究可以为通过调节肠道菌群分布对IgA肾病进行预防以及改善疾病预后等方面的进一步研究提供初步临床实验资料。

Objective Intestinal flora participates in human immune regulation by affecting intestinal mucosa and altered distribution and diversity of intestinal flora change the immune response of human body.This study preliminarily explained the distribution characteristics of intestinal flora in IgA nephropathy patients by comparing the distribution characteristics and differences of intestinal flora between IgA nephropathy patients and normal controls.Methods Fecal samples of patients diagnosed as IgA nephropathy undergoing kidney biopsy between October 2018 and October 2019 were collected as IgA group.And fecal samples from healthy people were collected as normal control group.Twenty samples from each group were submitted for biodiversity analysis,including OTU,species classification,Alpha diversity and Beta diversity,etc.Results Seven OTUs in IgA group were different from those in normal control group.LEfSe analysis between groups revealed that o_Enterobacteriales and f_Enterobacteriaceae had a great influence on the species difference of samples.From the perspective of species composition,the species composition of healthy control group was more abundant than that of IgA group,and species uniformity was also higher than that of IgAN patients.Conclusion The diversity of intestinal flora declines in patients with IgA nephropathy and there is also a change of dominant flora.This study provides preliminary rationales for further researches on preventing IgA nephropathy and improving disease prognosis through regulating the distribution of intestinal flora.