黄素依赖型卤化酶的结构特点及工程改造

The Structural Characteristics and Engineering Reconstruction of Flavin-dependent Halogenase

卤化物是通过卤化酶催化卤族元素在有机化合物上特定位置发生取代形成的一类化合物,具有独特的生理生化作用。黄素依赖型卤化酶具有良好的区域选择性,虽然有相似的黄素分子的结合位点,但在底物结合方面略有不同,对其结构和合成途径及结合蛋白质工程的随机诱变和定向改造的研究在工业应用中至关重要。讨论了具有高区域选择性的黄素依赖型卤化酶的结构特点及工程改造,以及经过工程改造后黄素依赖型卤化酶在工业生产中的应用。

Halides are a kind of compounds which catalyzed by halogenase to the addition of halogen in secondary metabolites,have unique physiological and biochemical functions.Flavin-dependent halogenases have good regional selectivity and stability,and similar FAD binding sites with slight difference in substrates made them very important in industrial application.Therefore,the studies on its structure and synthetic pathway as well as random mutagenesis and directed modification of protein engineering are very important for its industry application.In this paper,the structural characteristics and engineering modification of flavin-dependent halogenases with high regional selectivity are reviewed,and it is of guiding significance for the application of engineering-modification flavin-dependent halides in industrial production.