鸡GSTA2基因5′UTR区域变异及其与重要经济性状关联性

Variation of GSTA2 Gene in 5′UTR Region and Its Correlation with Important Economic Characters

试验旨在分析GSTA2基因的SNPs和杏花鸡与隐性白洛克鸡F2群体的肉质、生长和屠体性状的相关性。试验采用Sanger测序从359个杏花鸡与隐性白洛克鸡F2群体的基因组序列中筛选到4个GSTA2基因的SNPs,均位于5′UTR区域。利用SPSS 22.0软件对4个SNPs与杏花鸡和隐性白洛克鸡F2群体的经济性状进行了关联分析。结果显示:4个SNPs与杏花鸡和隐性白洛克鸡F2群体的体重、胫长、肤色等性状显著相关。对4个SNPs进行单倍型分析发现SNP1、SNP3、SNP4组成的单倍型AGAGTT与鸡的翅重有显著相关性,SNP2、SNP4组成的单倍型GGTT与鸡的胫长有显著相关性。结果表明GSTA2基因的SNPs显著影响鸡的经济性状,可以作为鸡经济性状选育的候选分子标记。

The aim of the experiment was to analyze the correlation between SNPs of GSTA2 gene and the weight,growth and carcass traits of the F2 population of Xinghua and White Recessive Rock. In the experiment, 4 SNPs were found in GSTA2 gene with sanger sequencing from the genome sequences of 359 F2 generation resources group,and all located in 5′UTR region. SPSS 22.0 software was used to analyze the correlation between 4 SNPs and the economic characters of the F2 population of Xinghua and White Recessive Rock. The results showed that 4 SNPs were significantly correlated with body weight, shank length and skin color of the F2 population of Xinghua and White Recessive Rock. Haplotype analysis of 4 SNPs showed that haplotype AGAGTT composed of SNP1, SNP3 and SNP4 was significantly correlated with chicken wing weight, while haplotype GGTT composed of SNP2 and SNP4 was significantly correlated with chicken shank length. These results indicated that these SNPs of GSTA2 gene had significant influence on the economic traits of chicken and could be used as candidate molecular markers for the breeding of chicken economic traits.