乌梅对抗生素所致小鼠肠道菌群失调的改善作用

Ameliorating effect of Mume Fructus on dysbiosis of murine gut microbiota induced by antibiotics

本研究旨在探讨药食同源中药乌梅对抗生素所致小鼠肠道菌群失调的改善作用,为乌梅的进一步开发利用提供科学依据。构建广谱抗生素诱导的小鼠肠道菌群紊乱模型,40只ICR小鼠随机分为空白对照、模型组、乌梅低、中、高剂量组,连续灌胃15天后,采集小鼠新鲜粪便样本。采用高通量测序技术对乌梅干预前后小鼠的肠道菌群进行16S rRNA基因测序,对测序结果进行生物信息学分析,并检测体质量和炎性因子改变。结果显示,中、高剂量乌梅可以显著降低小鼠体重;中剂量乌梅可以调节抗生素引起的TNF-α、IL-1β、IL-6的变化至正常水平;病理切片显示高剂量乌梅对胃有一定刺激作用。对菌群结构的分析表明乌梅可以部分改善抗生素引起的菌群紊乱的状况:在门水平上,中、低剂量的乌梅显著降低变形菌门的相对丰度;在属水平上,中、低剂量的乌梅显著上调Lactobacillus(P=0.001,P=0.000)和Faecalibaculum(P=0.005,P=0.038),高、低剂量的乌梅显著上调Bifidobacterium(P=0.000,P=0.000)和Blautia(P=0.049,P=0.000)的相对丰度。本研究从肠道微生态的视角为乌梅的临床应用提供科学依据,并揭示其作为肠道微生态调节剂产品的前景。

This study aimed to investigate the ameliorating effects of Mume Fructus(MF)on dysbiosis of murine gut microbiota induced by antibiotics,and to supply scientific basis for further development and utilization of Mume Fructus.Forty ICR mice were randomly divided into a blank group of 8 and an intestinal dysbiosis group of 32,which were randomly divided into model,high-dose MF(H),medium-dose MF(Me)and low-dose MF(L)groups.Thirty-two mice with intestinal dysbiosis received respectively treatment for 15 days,and simultaneously antibiotics(ig)from the tenth day.The murine weight was monitored,the pathological changes of tissues were observed by hematoxylin and eosin(HE)staining,and the murine gut miocrobiota determined by high-throughput sequencing and inflammatory factors detected by enzyme-linked immunosorbent assay(ELISA).Compared with normal group,H and Me groups reduced the average body weight and reversed the weight gain induced by antibiotics.The Me group restored the inflammatory markers,TNF-α,IL-1βand IL-6 to normal.Observations from a HE staining experiment demonstrated that H group had gastric irritation.At the phylum level,compared with the model group,the relative abundance of Proteobacteria had decreased in the L and Me groups.Meanwhile,at the genus level,the relative abundance of Lactobacillus and Faecalibaculum had increased in the L and Me groups,and those of Bifidobacterium and Blautia had increased in the L and H groups.This study provided a scientific basis for clinical medication of MF from the perspective of microecology,and revealed its potential as intestinal microecological modulator.