摘要
本实验对九蒸九晒熟地黄进行进一步的化学成分研究,采用多种柱色谱技术从“九蒸九晒”熟地黄70%丙酮提取物中分离得到3个紫罗兰酮类化合物,运用现代波谱技术并结合参考文献对其结构进行鉴定,分别为sec-hydroxyaeginetic acid-2-O-β-D-glucopyranoside(1)、frehmaglutin A(2)和dihydroxy-β-ionone(3)。其中化合物1为新化合物,化合物2和3为首次从熟地黄中分离得到。采用MTT法研究化合物1~3对人恶性黑色素瘤A375细胞的细胞毒作用。结果表明,化合物1~3在25μM浓度下均能显著的降低A375的细胞活力,提示其可能具有A375细胞毒活性。
Three ionones were isolated from the Rehmanniae Radix Praeparata,which were processed by the traditionally-adopted nine cycles of steaming and sun-drying,by using multiple chromatographic methods and their structures were elucidated as sec-hydroxyaeginetic acid-2-O-β-D-glucopyranoside,frehmaglutin A,dihydroxy-β-ionone,from their spectral data and physicochemical properties.Among them,compound 1 is a new ionone glycoside.Compounds 2 and 3 were isolated from the Rehmanniae Radix Praeparata for the first time.The effect of compounds 1-3 on Human malignant melanoma A375 cell was investigated by MTT.The results showed that compounds 1-3(25μM)significantly inhibited the cell activities of A375 cells.
作者
张靖柯
吕锦锦
李孟
魏俊俊
郑晓珂
冯卫生
ZHANG Jing-ke;LYU Jin-jin;LI Meng;WEI Jun-jun;ZHENG Xiao-ke;FENG Wei-sheng(The Engineering and Technology Center for Chinese Medicine Development of Henan Province of Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《天然产物研究与开发》
CAS
CSCD
2021年第5期767-772,共6页
Natural Product Research and Development
基金
国家重点研发计划中医药现代化研究重点专项(2017 YFC1702800)
河南省高层次人才特殊支持计划“中原千人计划”-中原领军人才(ZYQR201810080)。
关键词
熟地黄
化学成分
紫罗兰酮
细胞毒
Rehmanniae Radix Praeparata
chemical compositions
ionone
cytotoxicity