九蒸九晒熟地黄中的紫罗兰酮类化合物

Ionones from the“nine steaming nine sun-drying”Rehmanniae Radix Praeparata

本实验对九蒸九晒熟地黄进行进一步的化学成分研究,采用多种柱色谱技术从“九蒸九晒”熟地黄70%丙酮提取物中分离得到3个紫罗兰酮类化合物,运用现代波谱技术并结合参考文献对其结构进行鉴定,分别为sec-hydroxyaeginetic acid-2-O-β-D-glucopyranoside(1)、frehmaglutin A(2)和dihydroxy-β-ionone(3)。其中化合物1为新化合物,化合物2和3为首次从熟地黄中分离得到。采用MTT法研究化合物1~3对人恶性黑色素瘤A375细胞的细胞毒作用。结果表明,化合物1~3在25μM浓度下均能显著的降低A375的细胞活力,提示其可能具有A375细胞毒活性。

Three ionones were isolated from the Rehmanniae Radix Praeparata,which were processed by the traditionally-adopted nine cycles of steaming and sun-drying,by using multiple chromatographic methods and their structures were elucidated as sec-hydroxyaeginetic acid-2-O-β-D-glucopyranoside,frehmaglutin A,dihydroxy-β-ionone,from their spectral data and physicochemical properties.Among them,compound 1 is a new ionone glycoside.Compounds 2 and 3 were isolated from the Rehmanniae Radix Praeparata for the first time.The effect of compounds 1-3 on Human malignant melanoma A375 cell was investigated by MTT.The results showed that compounds 1-3(25μM)significantly inhibited the cell activities of A375 cells.