重组人生长激素对兔角膜损伤早期修复的研究

Effect of recombinant human growth hormone on corneal epithelial wound healing

目的:探讨重组人生长激素(rHGH)对兔角膜上皮损伤早期的修复作用。方法:选取32只健康雄性新西兰兔制作双眼角膜上皮损伤模型,随机选取1眼滴无菌生理盐水作为对照组,另1眼滴20nmol/L重组人生长激素作为rHGH组,分别于造模前、造模后24、48、72h采用荧光素钠染色法观察角膜上皮愈合情况,采用Cochet-Bonnet角膜知觉测量仪测量检查中央角膜敏感度,同时收集各时间点泪液检测炎症因子的表达。结果:造模后48h对照组和rHGH组角膜上皮愈合率分别为62.52%±6.73%和79.62%±10.62%(P<0.05),造模后72h分别为90.56%±9.57%和98.43%±3.65%(P<0.05)。造模后48h,对照组和rHGH组中央角膜敏感度分别为3.22±0.42、4.22±0.26cm(P<0.05)。造模后各时间点两组泪液TNF-α和IL-1α表达均较造模前增加,且对照组均高于rHGH组。造模后24、48h,两组泪液MMP-9表达均较造模前增加,且造模后48h对照组高于rHGH组(均P<0.05)。造模后24、48h两组泪液IL-21表达均较造模前增加(P<0.05)。造模后各时间点两组泪液IL-17α、Leptin表达与造模前相比及两组之间比较均无差异(P>0.05)。结论:重组人生长激素有利于兔角膜上皮损伤早期修复。

AIM:To explore the effects of recombinant human growth hormone(rHGH)on early rehabilitation in rabbit corneal epithelial wound.METHODS:Thirty-two New Zealand rabbits were selected to establish the corneal epithelial defects models.One eye was treated with normal saline(NC group)and the other eye was treated with 20nmol/L rHGH(rHGH group)in a randomized double-blinded way.The corneal healing process was monitored by the corneal fluorescein staining scores at 0h,24h,48h,and 72h after the surgery.The central corneal sensitivity was detected by Cochet-Bonnet corneal esthesiometer and the concentrations of inflammatory mediators interlecukin-1α(IL-1α),interlecukin-17(IL-17),interlecukin-21(IL-21),Leptin,matrix metalloproteinase-9(MMP-9)and tumor necrosis factor-α(TNF-α)in collected tears were measured by multiplex antibody microarray.RESULTS:The corneal epithelial healing rates of the NC group and rHGH group were(62.52±6.73)%and(79.62±10.62)%(P<0.05),(90.56±9.57)%and(98.43±3.65)%(P<0.05)at 48h and 72h postoperatively.The central corneal sensitivity of rHGH group(4.22±0.26)cm was better than that in NC group(3.22±0.42)cm at 48h after surgery(P<0.05).The expressions of TNF-αand IL-1αincreased in both groups at each time point after operation,and the expressions in NC group were higher than those in the rHGH group.Both groups had higher MMP-9 concentrations in the tear fluid at 24 and 48h postoperation in comparison with the point before the operation.The MMP-9 expression in NC group was higher than that in the rHGH group at 48h postoperatively.The expressions of IL-21 in NC group were higher than those in the rHGH group at 24 and 48h postoperation in comparison with the point before the operation(P<0.05).No significant differences in tearIL-17 and Leptin were observed between groups before and after surgery(P>0.05).CONCLUSION:Topical application of rHGH can accelerate the early stage of rabbit corneal epithelial wound healing in vivo.