人参皂苷溶液注射对小肠切除术后腹腔感染大鼠内皮素和一氧化氮的影响

Effects of ginsenosides solution injection on endothelin and nitric oxide in rats with abdominal infection after small bowel resection

目的探讨人参皂苷对小肠切除术后腹腔感染大鼠内皮素(ET)、一氧化氮(NO)的影响。方法将30只大鼠分为三组,为假手术组(进行小肠切除未做腹腔感染)、感染组(建立小肠切除合并腹腔感染大鼠模型)及人参皂苷组(建立小肠切除合并腹腔感染大鼠模型+人参皂苷溶液注射),采用HE染色观察病理切片,原位末端转移酶标记技术(TUNEL)法检测内皮细胞凋亡,检测血浆、组织中ET、NO水平及血清中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。结果假手术组大鼠小肠黏膜及绒毛发育良好,无炎性及黏膜病变;感染组大鼠绒毛水肿及紊乱,毛细血管出现充血现象;人参皂苷组小肠黏膜恢复正常,轻微上皮受损;注射7 d后,感染组TNF-α、IL-6水平高于假手术组(P<0.05),人参皂苷组上述指标水平低于感染组;注射7 d后,假手术组和人参皂苷组ET水平低于、NO水平高于感染组(P<0.05);注射7 d后,假手术组和人参皂苷组大鼠小肠组织中ET水平低于、NO水平高于感染组(P<0.001);三组大鼠小肠组织内皮细胞凋亡显示棕黑色,感染组内皮细胞凋亡率高于假手术组(P<0.05),人参皂苷组内皮细胞凋亡率低于感染组(P<0.05)。结论人参皂苷降低小肠切除合并腹腔感染大鼠炎症及ET水平,提高NO活性,改善病情。

OBJECTIVE To explore the effect of ginsenosides on ET and NO in rats with abdominal infection after small bowel resection.METHODS Thirty rats were divided into three groups:the sham operation group(without abdominal infection after small intestine resection),infection group(with abdominal infection after small intestine resection)and ginsenoside group(with abdominal infection after small intestine resection+ginsenoside solution injection).Pathological sections were observed by HE staining,endothelial cell apoptosis was detected by TUNEL method.The levels of ET,NO,IL-6 and TNF-αin serum and tissues was detected.RESULTS In the sham operation group,the small intestine mucosa and villi of rats were well developed,without inflammation and mucosal degeneration;in the infected group,villous were edema and disorders,and the capillaries became congested and in the ginsenoside group,the small intestine mucosa returned to normal,with slight epithelial damage.The levels of TNF-αand IL-6 in the infection group were significantly higher than those in other groups after 7 days of injection(P<0.05).And the above indexes were significantly lower than those in the infected group.After 7 days of gavage,ET activity was significantly lower,but NO levels was higher in the sham operation group than those in the infection group(P<0.05);ET level in the sham operation group and ginsenoside group was significantly lower than that in the infection group,and NO level was significantly higher than that in the infection group(P<0.001).The apoptosis of endothelial cells in the small intestine tissues in the three groups of rats were brown and black,and the apoptosis of endothelial cells in the infected group is significantly higher than hat in the sham operation group(P<0.05).Apoptosis of endothelial cells in the ginsenoside group was significantly lower than that in the infection group(P<0.05).CONCLUSION Ginsenoside reduced inflammation and ET level,increased NO activity and improved the condition of rats with small bowel resection and abdominal infection.