维生素D受体及IL-6基因多态性与儿童反复呼吸道感染的关系

Association between vitamin D receptor and IL-6 gene polymorphisms and recurrent respiratory tract infection in children

目的探究维生素D受体(VDR)、白介素-6(IL-6)基因多态性与儿童反复呼吸道感染(RRTI)的关系。方法选择2017年3月-2020年1月青海省妇女儿童医院收治的84例RRTI患儿作为RRTI组,选取同期体检的100名正常健康儿童作为对照组,均采血测定血清25-羟维生素D3[25(OH)D3]、IL-6水平,采用聚合酶链反应(PCR)-限制性内切酶片段长度多态性技术(RELP)检测VDR基因BsmI位点及IL-6基因rs1800796位点单核苷酸多态性(SNPs),分析以上基因多态性与儿童RRTI的关系。结果 RRTI组患儿血清25(OH)D3水平为(23.57±5.17)μg/L,低于对照组(29.45±6.36)μg/L(P<0.05),IL-6水平为(21.67±5.06)ng/L,高于对照组(4.15±1.16)ng/L(P<0.05);RRTI组携带B等位基因频率高于对照组,携带Bb基因型频率高于对照组(P<0.05);RRTI组携带C等位基因频率高于对照组,携带CC基因型频率高于对照组(P<0.05);携带VDR BsmI位点Bb基因型及等位基因B可使RRTI发生风险增加2.036倍与1.925倍(校正OR=2.036,95%CI=1.320~3.140;校正OR=1.925,95%CI=1.320~3.140);携带IL~6 rs1800796位点CC基因型及等位基因C可使RRTI发生风险增加1.586倍、2.136倍(校正OR=1.586,95%CI=1.028~2.445;校正OR=2.136,95%CI=1.385~3.294)。结论 VDR BsmI位点Bb基因型、等位基因B,IL-6 rs1800796位点CC基因型、等位基因C可能为RRTI易感基因型。

OBJECTIVE To explore the relationship between vitamin D receptor(VDR) and interleukin-6(IL-6) gene polymorphisms and recurrent respiratory tract infection(RRTI) in children. METHODS All 84 children with RRTI admitted to Qinghai Women’s and children’s Hospital between Mar. 2017 and Jan. 2020 were recruited and divided into the RRTI group;100 normal healthy children during the period were in the control group. Blood samples in both groups of children were collected to determine serum 25-hydroxyvitamin D3[25(OH) D3] and IL-6 levels. Polymerase chain reaction(PCR)-restriction enzyme fragment length polymorphism(RELP) was used to detect single nucleotide polymorphisms(SNPs) at VDR gene BsmI locus and IL-6 gene rs1800796 locus. The relationship between the above gene polymorphisms and RRTI in children was analyzed. RESULTS The serum 25(OH)D3 level in the RRTI group was(23.57±5.17) μg/L, which was significantly lower than that in the control group of(29.45±6.36) μg/L(P<0.05). The concentration of IL-6 was(21.67±5.06) ng/L, which was significantly higher than that in control group of(4.15±1.16) ng/L(P<0.05). The B allele frequency at BsmI locus in the RRTI group was significantly higher than that in the control group;and the Bb genotype frequency in the RRTI group was significantly higher than that in the control group(P<0.05). The C allele frequency and CC genotype frequency in the RRTI group was significantly higher than that in the control group(P<0.05). BsmI Bb genotype at locus of VDR gene and allele B increased the risk of RRTI by 2.036 times and 1.925 times(corrected OR=2.036, 95%CI=1.320-3.140, corrected OR=1.925, 95%CI=1.320-3.140). IL-6 rs1800796 locus CC genotype and allele C increased the risk of RRTI by 1.586 times and 2.136 times(corrected OR=1.586, 95%CI=1.028-2.445, corrected OR=2.136, 95%CI=1.385-3.294). CONCLUSION VDR BsmI locus Bb genotype and allele B, IL-6 rs1800796 locus CC genotype and allele C may be susceptible genotypes of RRTI.