百令胶囊对支气管哮喘小鼠的治疗效果

Therapeutic effect of Bailing capsule in a murine asthma model

目的探讨百令胶囊对支气管哮喘小鼠气道炎症、气道高反应和气道重塑的治疗效果。方法将48只雌性Balb/c小鼠按随机数字表法分为正常对照组、支气管哮喘组、布地奈德组、低剂量百令胶囊组、中剂量百令胶囊组、高剂量百令胶囊组,每组8只。测定各组小鼠的气道阻力,计数小鼠肺泡灌洗液(BALF)中细胞总数。HE、AB-PAS和Masson染色观察气道炎症、气道重塑。采用免疫组化染色评估各组小鼠肺组织内α-平滑肌肌动蛋白(α-SMA)水平。结果与支气管哮喘组相比,布地奈德组、低剂量百令胶囊组、中剂量百令胶囊组、高剂量百令胶囊组小鼠气道高反应、BALF中炎症细胞总数均降低(P<0.05);肺组织气道周围炎症减轻,杯状细胞化生降低,胶原沉积减少;α-SMA表达水平降低。低剂量百令胶囊组、中剂量百令胶囊组、高剂量百令胶囊组与布地奈德组比较,气道反应性无统计学差异(P>0.05)。除气道反应性外,布地奈德组上述指标均优于低剂量百令胶囊组、中剂量百令胶囊组、高剂量百令胶囊组。结论百令胶囊可以抑制哮喘小鼠气道炎症、气道高反应和气道重塑。

Objective To investigate the effects of Bailing capsule on the airway inflammation,remodeling,and resistance of mice with asthma.Methods 48 Balb/c mice were randomly divided into a control group,asthma group,budesonide group,and low-,middle-,and high-dose Bailing groups(n=8 per group).The airway resistance was measured,and total cells in the bronchoalveolar lavage fluid(BALF)were counted.HE,AB-PS,and Masson staining were performed to assess airway inflammation and remodeling.Immunohistochemical staining was performed to determine the expression ofα-smooth muscle actin(α-SMA).Results Compared with the asthma group,the airway resistance and the number of total inflammatory cells in BALF decreased in the budesonide group and in the low-,middle-,and high-dose Bailing groups(P<0.05).Meanwhile,the inflammatory cell infiltration,goblet cell metaplasia,collagen deposition,andα-SMA expression in lung tissue also decreased.No significant difference in airway resistance was found between the budesonide and Bailing groups(P>0.05).Except for airway resistance,the aforementioned effects in the budesonide group were better than those in all of the Bailing groups.Conclusion Bailing capsule can inhibit airway inflammation,resistance,and remodeling in asthmatic mice.