基于网络药理学探讨白芍治疗帕金森病的作用机制

Study on Mechanism of Baishao (Radix Paeoniae Alba) in Treatment of Parkinson’s Disease Based on Network Pharmacology

目的基于网络药理学探讨白芍治疗帕金森病(PD)的作用机制。方法在TCMSP分析平台检索中药活性成分和中药靶点,利用GeneCards数据库和OMIM数据库筛选白芍治疗PD的作用靶点,构建蛋白互作网络(PPI),进行GO功能富集分析及KEGG通路富集分析,构建可视化的中药-疾病靶点相关的信号通路。结果 (1)从白芍中筛选出5个有效成分,作用于61个帕金森病靶点,这5个有效成分是芍药苷元、芍药苷、β-谷甾醇、山柰酚、(+)-儿茶素。(2)白芍治疗PD的核心基因主要有IL6、AKT1、JUN、MAPK8、PTGS2等。(3)中药-疾病靶点涉及的GO生物学功能有107个,主要包括肽结合、酰胺结合、血红素结合、四吡咯结合、G蛋白偶联胺受体活性、神经递质受体活性、泛素样蛋白连接酶结合、乙酰胆碱受体活性、G蛋白偶联神经递质受体、单加氧酶活性、氧化还原酶活性等。(4)中药-疾病靶点涉及的KEGG通路有129条,主要包括AGE-RAGE信号通路、流体剪切应力与动脉粥样硬化通路、肿瘤坏死因子信号通路、结核病通路、卡波西肉瘤相关疱疹病毒感染通路、乙型肝炎病毒通路、EB病毒感染通路、麻疹通路、弓形体病通路、甲型流感病毒通路等。结论白芍是通过多组分、多靶点、多通路达到治疗PD目标的,基于网络药理学探讨白芍治疗PD的作用机制,为中药现代化研究提供新思路,为PD临床治疗提供理论依据。

Objective Based on the network pharmacology, to observe the mechanism of Baishao (Radix Paeoniae Alba) in the treatment of Parkinson’s disease (PD). Method In TCMSP analysis platform, the active ingredients in Chinese medicine and traditional Chinese medicine (TCM) targets were retrieved. GeneCards database and OMIM database were used for screening Baishao (Radix Paeoniae Alba) targets for the treatment of PD to construct the protein-protein interaction network (PPI),carry out the GO function enrichment analysis and KEGG pathway enrichment analysis and build up the visualization of traditional Chinese medicine-disease targets related signaling pathway. Results (1)There were five active ingredients extracted from Baishao (Radix Paeoniae Alba), acting on 61 targets for PD. The five active ingredients were paeoniflorin, paeoniflorin, β-sitosterol, rhizoma kaempferiae phenol and (+)-catechin. (2)The core genes of Baishao (Radix Paeoniae Alba) treating PD were mainly IL6, AKT1, JUN, MAPK8, PTGS2, etc. (3)The GO biological functions involved in disease-traditional Chinese medicine targets were 107, mainly including peptide combining, amide bond, hemoglobin, four pyrrole combination, G protein coupled receptor activity and activity of neurotransmitter receptors, ubiquitin protein ligase, acetylcholine receptor activity, G protein coupling of neurotransmitter receptors, single oxygenase activated, REDOX enzyme activity, etc. (4)Traditional Chinese medicine-disease targets involved 129 KEGG pathways, including AGE-RAGE signal pathway, fluid shear stress and atherosclerosis, tumor necrosis factor signaling pathways, tuberculosis (TB) pathway, Kaposi’s sarcoma associated herpesvirus infection pathway, hepatitis B virus, epstein-barr virus infection pathways, measles pathway, toxoplasmosis, influenza A virus pathways, etc. Conclusion radix paeoniae alba by multicomponent, multiple targets, multiple pathways to achieve the goal of treatment of PD, based on the network pharmacology study the mechanism of action of radix paeoniae alba treatment of PD, provide new ideas for the research on traditional Chinese medicine (TCM) modernization, as to provide theoretical basis for clinical application of PD.