人参皂苷Rg1对环磷酰胺致成年雄性小鼠睾丸生精细胞凋亡的保护作用

Protective Effect of Ginsenoside Rg1 on Cyclophosphamide-Induced Apoptosis of Testicular Spermatogenic Cells in Adult Male Mice

目的探讨环磷酰胺致成年雄性小鼠睾丸生精细胞凋亡模型经人参皂苷Rg1干预后的效果。方法选择小鼠50只,经环磷酰胺造模后,分为5组:空白对照组、模型组、人参皂苷Rg1低、中、高剂量组,剂量:5、10、20 mg/kg, 10只每组。环磷酰胺造模5 d后,以人参皂苷Rg1(剂量5、10、20 mg/kg)灌胃干预,1次/d,连续2周;空白及模型对照组给予双蒸水,时间同前。在总治疗的第30天处死小鼠,依实验设计检测小鼠睾丸指数、睾丸组织形态、睾丸生精细胞凋亡、钙敏感受体(CaSR) mRNA及CaSR蛋白表达。结果空白对照组体质量、睾丸指数、睾丸质量高于模型组;给药后,人参皂苷Rg1各剂量组中的指标高于模型组(P<0.05)。空白对照组、人参皂苷各剂量组的A级、A+B级精子的百分比、总活力、浓度及总数均高于模型组(P<0.05)。人参皂苷Rg1低中高剂量组中小鼠睾丸组织中的生精细胞排列整齐有序,且数量随人参皂苷Rg1剂量增加而增加。人参皂苷Rg1干预环磷酰胺致成年雄性小鼠睾丸生精细胞凋亡后,显著逆转生精细胞内Wnt2、CaSR mRNA的表达(P<0.05)。同时,人参皂苷Rg1干预后,生精细胞内CaSR蛋白的表达也得到显著性逆转(P<0.05)。结论人参皂苷Rg1对环磷酰胺致成年雄性小鼠睾丸生精细胞凋亡具有明显保护作用,其机制可能与上调CaSR蛋白的表达有关。

Objective To investigate the effect of ginsenoside Rg1 on cyclophosphamide-induced apoptosis of testicular spermatogenic cells in adult male mice. Methods Fifty mice were selected and divided into five groups: blank control group, model group, low, medium and high dose groups of ginsenoside Rg1 (with doses of 5,10,20 mg/kg), 10 mice in each group. After 5 days of cyclophosphamide modeling, ginsenoside Rg1 (5, 10, 20 mg/kg) was administered orally once a day for two consecutive weeks. The blank and model control groups were given double steaming water at the same time. On the 30 th day of the total treatment, mice were executed, and testicular index, testicular histomorphology, apoptosis of testicular spermatogenic cells, CaSR mRNA and CaSR protein expression were detected according to the experimental design. Results The body mass, testicular index and testicular quality of the blank control group were higher than those of the model group. After administration, the indexes of ginsenoside Rg1 in each dose group were higher than those before administration (P<0.05). The spermatogenic cells in the testicular tissue of mice in the low, middle and high dose groups of ginsenoside Rg1 were arranged orderly, and the number of spermatogenic cells increased with the increase of ginsenoside Rg1 dose. Ginsenoside Rg1 significantly reversed the expressions of Wnt2 and CaSR in spermatogenic cells of adult male mice after interfering with cyclophosphamide-induced spermatogenic cell apoptosis (P<0.05). At the same time, the expression of CaSR protein in spermatogenic cells was significantly reversed after ginsenoside Rg1 intervention (P<0.05). Conclusion Ginsenoside Rg1 has significant protective effect on cyclophosphamide-induced apoptosis of testicular spermatogenic cells in adult male mice, and its mechanism may be related to up-regulation of CaSR protein expression.