液相色谱-串联质谱法测定凉茶中非法添加的26种止咳平喘类化学药物

Determination of 26 Chemical Drugs for the Treatment of Cough and Asthma Added Illegally in Herbal Tea by High Performance Liquid Chromatography-tandem Mass Spectrometry

目的建立凉茶中26种止咳平喘类化学药物的快速筛查方法。方法采用液相色谱-串联质谱法,样品经甲醇提取,经0.22μm微孔滤膜过滤,以5 mmol·L^(-1)甲酸铵(0.1%甲酸)水溶液-乙腈溶液作为流动相进行洗脱,用C18(2.1 mm×150 mm,3.5μm)色谱柱分离,多反应监测(MRM)模式进行定性和定量分析。结果26种化学药物线性相关系数均>0.995,平均回收率为61.2%~115.6%,相对标准偏差为1.0%~2.0%(n=6)。结论该方法简单、快速、灵敏,可用于凉茶中非法添加的26种止咳平喘类化学药物的检测。

OBJECTIVE To establish a rapid screening method for determination of 26 chemical drugs for the treatment of cough and asthma added illegally in herbal tea.METHODS This method involved liquid chromatography-tandem mass spectrometry.The samples were extracted with methanol,filtrated by 0.22μm microporous filters and finally separated on a C18 column(2.1 mm×150 mm,3.5μm)by using acetonitrile and water(containing 5 mmol·L^(-1) ammonium formate and 0.1%formic acid)as mobile phase.Qualitative and quantitative analysis were carried out in multiple reaction monitoring(MRM)model.RESULTS The correlative coefficient of 26 chemical drugs were above 0.995.The average recoveries were 61.2%~115.6%,the relative standard deviations ranged from 1.0%to 2.0%(n=6).CONCLUSION The method is simple,rapid and sensitive.It is suitable for the determination of 26 chemical drugs for the treatment of cough and asthma added illegally in herbal tea.