玉米TPP家族基因生物信息学及表达分析

Bioinformatics and Expression Analysis of TPP Family Genes in Maize

TPP是合成海藻糖的关键酶,广泛存在于各种植株中,可通过影响海藻糖的合成参与调控植株的抗逆体系。分析玉米中TPP家族基因的性质和进化有利于进一步为玉米在逆境中的产量品质提高的研究奠定基础。本研究利用拟南芥的9个TPP家族基因,结合BLAST、Pfam、ExPASy、MEGA 6.0、MEME、TBtools等软件和网站,对玉米TPP家族成员的基因位置、基因结构、蛋白质理化性质、保守基序、系统进化和基因表达模式进行分析。结果显示,根据B73的完整基因组数据,在玉米基因组中共鉴定了26个TPP基因,氨基酸数介于243和1 391之间,均为亲水性蛋白,且64.5%的成员属酸性蛋白。除第10号染色体外,玉米TPP家族基因在其余染色体上均有分布。鉴定了3个保守结构域,结合基因进化和基因结构分析,具有相同motif的基因在进化和基因结构上相对一致。根据基因启动子顺式作用元件分析,ZmTPP11可能与提高植株低温抗性有关,ZmTPP15可能与加速玉米机械损伤愈合有关。通过对玉米TPP基因表达模式分析,发现有5个TPP基因在各组织和发育时期均高表达,而共表达分析和GO富集分析显示与ZmTPPs共表达的基因主要参与应激反应、信号传导、发育过程和生物调节等生物过程。研究结果为进一步研究玉米对逆境胁迫的响应和生长发育提供参考。

TPP is a key enzyme for trehalose biosynthesis, which wildly exists in various plants, and can be involved in regulating the stress tolerance system of plants by affecting trehalose synthesis. Analysis the characters and evolution of TPP family genes in maize will be helpful to further study on yield and quality improvement in maize under adversity. In present study, we used the nine TPP genes from Arabidopsis thaliana, and combined some bioinformatics software and website, such as BLAST, Pfam, ExPASy, MEGA 6.0, MEME, TBtools and so on, to analyze the gene location, gene structure, physicochemical properties of protein, motif, phylogenetic tree,and gene expression pattern and network. The results shown that 26 ZmTPP genes were identified and characterized based on complete genome data of B73, the amino acid numbers of 26 ZmTPPs were between 243 and 1391.All of them were hydrophilic proteins, and 64.5% of them were acidic proteins. Gene Mapping revealed that TPP family genes exited on all chromosomes except chromosome 10. There are three conserved domains in TPP family members of maize. Combined with different analyses, we found the genes with the same motif were similar in evolution and same in gene structure. According to the analysis of gene promot er cis-regulatory element, ZmTPP11 may be related to the improvement of plant low temperature resistance, and ZmTPP15 may be related to the acceleration of mechanical injury healing in maize. Through the gene expression pattern analysis of maize TPP genes,we found that 5 ZmTPPs with high expression level in different tissues and development periods. The co-expression and GO term analysis shown that the co-expression genes with ZmTPPs significant enrichment in terms relating to response to stimulus, signaling, developmental process, biological regulation, and other process. Our results provided candidate genes for further research on the stress response and developmental process of maize.