落叶松针叶直接不定芽发生体系的建立

Direct Regeneration of Adventitious Bud from Needles of Larix

本研究以落叶松针叶为起始外植体,开展直接不定芽诱导、伸长及不定根诱导等研究,建立了落叶松针叶直接不定芽发生体系,为该物种基因工程育种、基因编辑及优质种苗繁育等提供了全新途径。结果表明,DCR+2 mg/L 6-BA+0.2 mg/L NAA为不定芽诱导的较佳配方,不定芽诱导率达66.33%,平均芽数80个/外植体;伸长分为DCR+0.1 mg/L 6-BA+0.01 mg/L NAA和DCR+0.1 mg/L 6-BA+0.01 mg/L NAA+2 g/L活性炭两个步骤进行,不定芽伸长率达到60%,平均芽苗长度为2.57 cm;DCR+3 mg/L IBA+0.02 mg/L烯效唑为较佳的不定根诱导培养配方,不定根诱导率达到53.33%,移栽成活率达到87.48%。本研究可为落叶松基因工程育种、基因编辑及优质种苗繁育等研究与应用提供基础。

In this study, larix needles were used as the initial explants to carry out direct adventitious bud induction, elongation and adventitious root induction, and established a direct adventitious bud generation system for larix needles, which provided a new way for genetic engineering breeding, gene editing and high-quality seedling breeding of this species. The results showed that DCR +2 mg/L 6-BA +0.2 mg/L NAA was a better formula for adventitious bud induction, the induction rate of adventitious buds was 66.33%, and the average bud number was80 per explant. The elongation was divided into DCR+0.1 mg/L 6-BA+0.01 mg/L NAA and DCR+0.1 mg/L 6-BA+0.01 mg/L NAA+2 g/L AC, the elongation rate of adventitious shoots reached 60%, and the average shoot length was 2.57 cm. DCR+3 mg/L IBA+0.02 mg/L sacroconazole was a better adventitious root induction formula, the adventitious root induction rate reached 53.33%, and the transplant survival rate reached 87.48%. This research can provide a foundation for the research and application of larch genetic engineering breeding, gene editing and high-quality seedling breeding.