空肠弯曲菌多重毒力基因检测方法的建立

Establishment of detection methods for multiple virulence genes of Campylobacter jejuni

为了快速检测食品中空肠弯曲菌,应用聚合酶链式反应(polymerase chain reaction,PCR)对空肠弯曲菌保守序列(mapA)和细胞毒素调节基因(cdtB)预扩增后得到的双链核酸进行胶体金试纸条免疫检测,随着被检物中空肠弯曲菌浊度的增大,胶体金试纸条上的保守序列及其毒力基因检测线强度也呈梯度式变强。结果表明,空肠弯曲菌中mapA及cdtB的检出限均为10^(1) CFU/mL,实际样品的检测结果也呈现出极好的梯度。表明该方法具有灵敏度高、特异性好、简单快速等特点,为食品及环境中空肠弯曲菌的检测提供了一种新方法。

In order to rapidly detect Campylobacter jejuni in food,the conservative sequence(mapA)and cytotoxin regulatory gene(cdtB)of Campylobacter jejuni were pre-amplified by polymerase chain reaction(PCR)and double-stranded nucleic acid was obtained for immunoassay on colloidal gold strip.With the increase of the concentration of Campylobacter jejuni,the conservative sequence on colloidal gold strip and the strength of its virulence gene detection line were detected.It also became stronger in a gradient manner.The results showed that the detection limits of mapA and cdtB in Campylobacter jejuni were 10^(1) CFU/mL,and the detection results of actual samples also showed excellent gradient.The results show that the method is sensitive,specific,simple and fast,and provides a new method for the detection of Campylobacter jejuni in food and environment.