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毛花猕猴桃LEAFY基因克隆及表达模式分析

Cloning and expression pattern of LEAFY gene in Actinidia eriantha Benth.
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摘要 LEAFY(LFY)基因是开花信号途径中的最关键整合子,其在花序和花发育的各个阶段都起着至关重要的作用。文章以猕猴桃全基因组数据库为基础,选取毛花猕猴桃(Actinidia eriantha Benth.)‘华特’为实验材料,通过同源序列比对分析,从‘华特’猕猴桃基因组中得到了1个LFY同源基因,命名为AeLFY-like1。生物信息学分析显示,AeLFY-like1基因全长2432 bp,含有1095 bp的完整的编码框,编码364个氨基酸。AeLFY-like1基因亚细胞定位在细胞核中,在花芽组织中表达量最高,表明可作为转录因子调控植物开花过程。LFY基因在进化过程中高度保守,在不同物种中具有类似的结构和功能。该研究为进一步了解猕猴桃LFY基因功能与系统进化提供科学依据。 LEAFY(LFY)gene is the most critical integron in flowering signaling pathway,which plays an important role in inflorescence and flower development.Based on the whole genome database of Actinidia chinensis,Actinidia eriantha Benth.‘White’was used as the experimental material.Through homologous sequence alignment analysis,LFY homologous gene from Actinidia eriantha Benth.‘White’was obtained,named AeLFY-like1.Bioinformatics analysis showed that AeLFY-like1 gene was 2432 bp in length and contained a complete coding frame of 1095 bp,encoding 364 amino acids.AeLFY-like1 gene locates in the nucleus and has the highest expression in flower bud tissue,indicating that it may function as a transcription factor to regulate plant flowering process.LFY gene is highly conserved during evolution and has similar structure and function in different species.This study provides a scientific basis for further understanding on the function and phylogenetic evolution of LFY gene in kiwifruit.
作者 刘现稳 朱舟 刘成龙 张立群 韦韩忠 唐维 LIU Xianwen;ZHU Zhou;LIU Chenglong;ZHANG Liqun;WEI Hanzhong;TANG Wei(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)
出处 《合肥工业大学学报(自然科学版)》 CAS 北大核心 2021年第5期706-710,共5页 Journal of Hefei University of Technology:Natural Science
基金 国家自然科学基金资助项目(31700266) 合肥工业大学2017年国家级大学生创新创业训练计划资助项目(201710359054)。
关键词 毛花猕猴桃 LFY基因 基因克隆 基因功能 系统进化 Actinidia eriantha Benth. LFY gene gene cloning gene function phylogenetic evolution
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