有机物对SNAD脱氮性能及微生物群落的影响

Effect of Organic Matter on Nitrogen Removal Performance and Microbial Communities in SNAD

以葡萄糖作为有机碳源的模拟废水为处理对象,采用序批式生物膜反应器(SBBR),考察不同COD对同步亚硝化、厌氧氨氧化和反硝化(SNAD)工艺脱氮性能的影响。结果表明,SNAD工艺的脱氮除性能随着COD/ρ(NH_(4)^(+)-N)的增加呈逐渐增强趋势,COD/ρ(NH_(4)^(+)-N)为0.80时,TN平均去除率从85.00%增至96.86%,COD平均去除率为82.81%。运行120 d后,生物膜中与脱氮相关的浮霉菌门和变形菌门相对丰度分别从56.44%和13.67%增至62.71%和16.00%。系统中仅检出一种厌氧氨氧化菌(AnAOB)为CandidatusJettenia,葡萄糖对CandidatusJettenia有显著促进作用,相对丰度从44.88%大幅增至62.33%,但却对SM1A02有极强的抑制作用,相对丰度从14.45%降至0.19%。反硝化菌(DNB)为Denitratisoma,相对丰度从4.08%增至10.17%。

Effect of different COD on nitrogen removal performance in simultaneous nitrification,anammox and denitrification(SNAD)process for the treatment of simulated wastewater with glucose as the organic carbon source was investigated in a sequencing biofilm batch reactor(SBBR).The results showed that the nitrogen removal performance of SNAD process was gradually enhanced with the increase of COD/ρ(NH_(4)^(+)-N).When the COD/ρ(NH_(4)^(+)-N)was 0.80,the average removal rate of TN increased from 85.00%to 96.86%,and the average removal rate of COD was 82.81%.After 120 days of operation,the relative abundances of Planctomycetes and Proteobacteria associated with nitrogen removal in biofilm increased from 56.44%and 13.67%to 62.71%and 16.00%,respectively.Only one type of AnAOB was detected as CandidatusJettenia in the system.Glucose significantly promoted CandidatusJettenia,and the relative abundance increased from 44.88%to 62.33%,but it had a strong inhibition on SM1 A02,and the relative abundance decreased from 14.45%to 0.19%.Denitrifying bacteria(DNB)was Denitratisoma and its relative abundance increased from 4.08%to 10.17%.