34%氟唑菌酰羟胺·咯菌腈悬浮剂的高效液相色谱分析

Analysis of Pydiflumetofen·Fludioxonil 34% SC by HPLC

[目的]建立了一种利用高效液相色谱同时测定34%氟唑菌酰羟胺·咯菌腈悬浮剂中各组分有效成分的分析方法。[方法]采用Poroshell 120 EC-C18反相色谱柱,以乙腈-0.1%磷酸水(体积比70∶30)为流动相,在检测波长230 nm条件下测定待测试样中氟唑菌酰羟胺和咯菌腈的含量。[结果]氟唑菌酰羟胺和咯菌腈的R2值均为1.00,RSD分别为7.0%和4.2%,变异系数为0.55%和0.19%,平均回收率分别为99.78%和100.10%。[结论]该方法分离效果好、精密度高、高效和准确性较好、线性相关性好,可用于34%氟唑菌酰羟胺·咯菌腈悬浮剂的定量分析。

[Aims]This study aims to establish an analytical method for the determination of the active constituents of each component in pydiflumetofen·ludioxonil 34%SC by HPLC.[Methods]Using Poroshell 120 EC-C18 reversed-phase chromatographic column,using acetonitrile-0.1% phosphoric acid water(70:30,by vol)as the mobile phase,the content of fluconazole and fludioxonil in the sample was determined under the detection wavelength of 230 nm.[Results]The linear correlation coefficients of pydiflumetofen and fludioxonil were 1.00,the standard deviations were7.0 and 4.2%,respectively,and the coefficients of variation were 0.55 and 0.19%.The average recovery rates were 99.78 and 100.10%,respectively.[Conclusions]This method has good separation effect,high precision,high efficiency and accuracy,good linear correlation,and can be used for the quantitative analysis of pydiflumetofen·ludioxonil 34% SC.