西鄂尔多斯珍稀植物绵刺的避旱相关基因研究

Study on Drought Avoidance Associated Genes in Rare Plants Potaninia Mongolica in West Ordos

为探究西鄂尔多斯自然生境下绵刺(Potaninia mongolica)转录组水平的干旱胁迫响应,利用转录组Illumina HiSeq X-ten测序平台,对绵刺(P.mongolica)不同生长时期进行转录组测序并对筛选出的耐旱相关基因进行分析。结果表明:转录组测序经质量控制后共得到75.63 Gb Clean Data,经组装后获得435276条Transcript和141608条Unigene。有87707个Unigene在八个功能数据库中获得了注释信息。通过对绵刺不同时期差异表达基因的GO富集分析发现,差异表达基因主要富集在GO三大类别中的55个分支;KEGG功能富集分析发现,差异表达基因主要集中于核糖体生物合成、光合作用、光合作用-天线蛋白等代谢通路。随着干旱胁迫加剧在生长期与休眠期,经筛选发现有37条Unigene与绵刺抗旱相关的功能基因,8条抗旱相关的转录因子,主要来自AP2/ERBP、NAC、bHLH、MYB/MYC转录因子家族。综合分析说明绵刺响应干旱胁迫是一个多基因、多个生物过程联合调控的结果。

The Illumina HiSeq X-ten system was utilized to carry out the transcriptome sequencing of P.mongolica in different growth stages for research of the mechanism of response to drought stress of P.mongolica in natural habitat of the west Ordos and identification of the drought tolerance associated genes in it.A total of 75.63 Gb clean data,435276 assembled transcripts and 87707 unigenes annotated from eight functional databases were obtained by the transcriptome sequencing.GO enrichment analysis found that differentially expressed genes were mainly enriched in 55 branches of three categories,and functional enrichment analysis by KEGG showed that differentially expressed genes were mainly concentrated in ribosomal biosynthesis,photosynthesis,photosynthesis-antenna protein and other metabolic pathways.Under enhanced drought stress intensity and dormant condition,37 Unigenes associated drought tolerance were found,in which 8 transcription factors were included,mainly belonging AP2/ERBP,NAC,bHLH and MYB/MYC transcription factor families.The research revealed that the response of P.mongolica to drought stress involved different genes and a variety of biological processes.