微小染色体维持蛋白4在胰腺导管腺癌中的表达及其与预后相关性研究

Expression of minichromosome maintenance protein 4 in pancreatic ductal adenocarcinoma and its correlation with prognosis

目的检测微小染色体维持蛋白4(MCM4)在胰腺导管腺癌组织中的表达情况,初步探究MCM4与胰腺导管腺癌的临床预后关系。探讨MCM4作为胰腺导管腺癌潜在的生物标志物的可能性。方法通过生物信息学的方法分析MCM4在胰腺导管腺癌组织及癌旁正常组织中的mRNA水平,并分析其表达与胰腺导管腺癌患者的生存率间的关系。回顾性分析76例接受手术治疗的胰腺导管腺癌患者的临床病理资料。采用免疫组化法检测胰腺导管腺癌组织和癌旁正常组织中的MCM4蛋白表达水平,分析其与胰腺导管腺癌患者临床病理特征的关系。结果生物信息学分析结果显示,MCM4的mRNA在胰腺导管腺癌组织中显著高表达,并与患者总生存率和无病生存率显著相关(均P<0.05)。免疫组化结果表明,MCM4在胰腺导管腺癌组织中的表达明显高于癌旁正常组织。MCM4在胰腺导管腺癌组织中的高表达与肿瘤大小相关(P=0.038),而与年龄、性别、肿瘤分级无关(均P>0.05)。结论胰腺导管腺癌组织中MCM4呈显著高表达并提示不良预后,且其表达水平与胰腺导管腺癌患者肿瘤大小有关。MCM4可作为胰腺导管腺癌的一个新的潜在生物标志物。

Objective To detect the expression of microchromosome maintenance protein 4(MCM4)in pancreatic ductal adenocarcinoma,and to explore the clinical prognosis of MCM4 and pancreatic ductal adenocarcinoma.To explore the possibility of MCM4 as a potential biomarker for pancreatic ductal adenocarcinoma.Methods The mRNA level of MCM4 in pancreatic ductal adenocarcinoma and normal tissues adjacent to the cancer was analyzed by bioinformatics methods,and the relationship between its expression and the survival rate of patients with pancreatic ductal adenocarcinoma was analyzed.The clinicopathological data of 76 patients with pancreatic ductal adenocarcinoma who underwent surgical treatment were retrospectively analyzed.Immunohistochemical method was used to detect the expression level of MCM4 protein in pancreatic ductal adenocarcinoma tissue and normal tissues adjacent to the cancer,and to analyze its relationship with clinicopathological characteristics of patients with pancreatic ductal adenocarcinoma.Results The results of bioinformatics analysis showed that MCM4 mRNA was significantly highly expressed in pancreatic ductal adenocarcinoma tissue,and was significantly correlated with the overall survival rate and disease-free survival rate of patients(all P<0.05).Immunohistochemical results showed that the expression of MCM4 in pancreatic ductal adenocarcinoma tissue was significantly higher than that in normal tissues adjacent to the cancer.The high expression of MCM4 in pancreatic ductal adenocarcinoma tissue was related to tumor size(P=0.038),but not related to age,gender,and tumor grade(all P>0.05).Conclusions MCM4 is highly expressed in pancreatic ductal adenocarcinoma tissues and indicates a poor prognosis,and its expression level is related to the tumor stage of pancreatic ductal adenocarcinoma.MCM4 may serve as a new potential biomarker for pancreatic ductal adenocarcinoma.