金荞麦黄酮生物合成途径分析及关键酶基因鉴定

Identification of potential genes involved in biosynthesis of flavonoid and analysis of biosynthetic pathway in Fagopyrum dibotrys

为了丰富金荞麦Fagopyrum dibotrys属植物的转录组数据,解析参与黄酮生物合成途径的关键酶基因,挖掘其功能基因,该研究利用BGISEQ-500测序平台对金荞麦的根状茎、根、花、叶和茎进行转录组测序,经过de novo从头组装转录本,共产生了205619条unigenes,132372条获得7个公共数据库功能注释。其中81327条unigenes比对注释到GO数据库,大部分unigenes注释在细胞过程、生物调控、结合和催化活性,86922条unigenes富集在136个KEGG代谢途径,鉴定了82条unigenes参与编码黄酮类生物合成10个关键代谢酶。对金荞麦根状茎与根、花、叶和茎的差异表达基因(DEGs)进行分析,获得了27962条共表达DEGs,有23515条根状茎组织特异性表达DEGs富集132条KEGG代谢途径,而13条unigenes显著富集在黄酮和黄酮醇生物合成。此外,分析鉴定了3427条unigenes参与编码60个转录因子(TFs)家族,有4条bHLH TFs富集在黄酮生物合成。该研究丰富了金荞麦属植物的转录组数据库,为植物黄酮生物合成关键酶的解析提供了参考,有助于从基因水平上进一步研究金荞麦黄酮生物合成关键酶的功能和调控机制。

In order to enrich the transcriptome data of Fagopyrum dibotrys plants,analyze the genes encoding key enzyme involved in flavonoid biosynthesis pathway,and mine their functional genes,in this study,we performed RNA sequencing analysis for the rhizomes,roots,flowers,leaves and stems of F.dibotrys on the BGISEQ-500 sequencing platform.After de novo assembly of transcripts,a total of 205619 unigenes were generated and 132372 unigenes were obtained and annotated into seven public databases,of which,81327 unigenes were mapped to the GO database and most of the unigenes were annotated in cellular process,biological regulation,binding and catalytic activity.Besides,86922 unigenes were enriched in 136 pathways using KEGG database′and we identified 82 unigenes that encodes key enzymes involved in flavonoid biosynthesis.Comparing rhizome with root,flower,leaf or stem in F.dibotrys,27962 co-expressed differentially expressed genes(DEGs)were obtained.Among them,23515 DEGs of rhizome tissue-specific were enriched into 132 pathways and 13 unigenes were significantly enriched in biosynthesis of flavone and flavonol.In addition,we also identified 3427 unigenes encoding 60 transcription factor(TFs)families as well as four unigenes encoding bHLH TFs were enriched in flavonoid biosynthesis.Our results greatly enriched the transcriptome database of plants,provided a reference for the analysis of key enzymes involved in flavonoid biosynthesis in plants,and will facilitate the study of the functions and regulatory mechanisms of key enzymes involved in flavonoid biosynthesis in F.dibotrys at the genetic level.