熏鲁香、乳香挥发油成分GC-MS分析及幽门螺杆菌抑菌活性研究

GC-MS analysis of volatile oil components of Mastiche and Olibanum and study on antibacterial activity of Helicobacter pylori

以熏鲁香、乳香药材为研究对象,采用水蒸气蒸馏法提取药材挥发油,通过GC-MS技术对挥发油化学成分进行分析,对比研究两者挥发油组分差异,并进行幽门螺杆体外抑菌活性研究。结果显示,熏鲁香、乳香挥发油得率分别为11.93%,2.40%;熏鲁香挥发油共分析鉴定出46个化合物,占挥发油总成分的91.31%,乳香挥发油共分析鉴定出35个化合物,占挥发油总成分的92.49%;对各组分进行分类对比研究,熏鲁香挥发油中单萜质量质量分数最高(40.69%),其次为醇类(28.48%),乳香挥发油中醇类质量质量分数最高(35.81%),其次为酯类(24.92%),两者挥发油成分存在较大差异,其化学成分差异可能是其功效应用差异的原因之一。体外抑菌实验表明,熏鲁香挥发油对幽门螺杆菌体外最小抑菌浓度(MIC)为1 mg·mL^(-1),乳香挥发油对幽门螺杆菌体外最小抑菌浓度(MIC)大于1 mg·mL^(-1),结合出油率结果,熏鲁香更具有抑制幽门螺杆菌活性优势。研究结果为熏鲁香、乳香合理应用提供科学依据。

The volatile oil from Mastiche and Olibanum medicinal materials was extracted by steam distillation,and the chemical components of the volatile oil were analyzed by GC-MS technology.The differences of the volatile oil components were compared and study on the Helicobacter pylori in vitro antimicrobial activitiy was conducted.The results showed that the yields of the volatile oil from Mastiche and Olibanum were 11.93%and 2.40%,respectively.A total of 46 compounds(91.31%)were identified from the volatile oil from Mastiche annd 35 compounds(92.49%)from Olibanum.The classification and comparison study of the components showed that the content of monoterpenes in the volatile oil from Mastiche was the highest(40.69%),followed by alcohols(28.48%);while the content of alcohols in the volatile oil from Olibanum was the highest(35.81%),followed by esters(24.92%).There were significant differences in the components of volatile oil from Mastiche and Olibanum,which might be one of the reasons for the difference in efficacy and application.In vitro bacteriostatic experiments showed that the minimum inhibitory concentration(MIC)of the volatile oil from Mastiche against H.pylori was 1 mg·mL^(-1),and the MIC of the volatile oil from Olibanum against H.pylori was more than 1 mg·mL^(-1).In combination with the results of the oil yield experiment,Mastiche had the advantage of inhibiting H.pylori activity.The research results provide scientific basis for the rational application of Mastiche and Olibanum.