青蒿的化学成分研究Ⅰ

Research on chemical constituents from Artemisia annuaⅠ

综合应用HP-20、硅胶、ODS、Sephadex LH-20、HW-40和半制备液相等多种色谱学方法对黄花蒿Artemisia annua干燥地上部分水提取物中的化合物进行分离纯化,并根据化合物的理化性质和核磁共振波谱数据进行结构鉴定。结果分离鉴定了15个化合物,分别为vitexnegheteroin M(1)、sibricose A5(2)、securoside A(3)、citrusin D(4)、annphenone(5)、E-melilotoside(6)、马栗树皮素(7)、东莨菪素苷(8)、刺五加苷B1(9)、猫眼草酚苷D(10)、万寿菊素-3-O-β-D-吡喃葡萄糖苷(11)、槲皮素-7-O-β-D-葡萄糖苷(12)、芦丁(13)、芹菜素-6,8-二葡萄糖碳苷(14)、异夏佛塔苷(15),其中化合物1~4为首次从蒿属植物中分离得到。采用LPS诱导小鼠巨噬细胞RAW264.7模型,以抑制炎性细胞因子前列腺素E2(PGE2)的分泌为评价指标对分离得到的化合物进行体外抗炎活性评价。结果显示化合物1,2,8,10~15对LPS诱导小鼠巨噬RAW264.7细胞中PGE2的分泌具有一定的抑制作用。

Chemical constituents were isolated and purified from the water extract of Artemisia annua by column chromatography of HP-20 macroporous resin,silica gel,ODS,Sephadex LH-20,HW-40,and semi-preparative RP-HPLC.Their structures were elucidated by physicochemical properties and spectral analyses.As a result,Fifteen compounds were isolated and identified as vitexnegheteroin M(1),sibricose A5(2),securoside A(3),citrusin D(4),annphenone(5),E-melilotoside(6),esculetin(7),scopoletin-7-O-β-D-glucoside(8),eleutheroside B1(9),chrysosplenol D(10),patuletin-3-O-β-D-glucopyranoside(11),quercetin-7-O-β-D-glucoside(12),rutin(13),apigenin 6,8-di-C-β-D-glucopyranoside(14),isoschaftoside(15),among them,compounds 1-4 were identified from Artemisia for the first time.Additionally,the isolates were evaluated for their inhibitory effects on the production of PGE2 in LPS-simulated RAW264.7 macrophages.The results showed that compounds 1,2,8,and 10-15 could reduce PGE2 levels,to a certain extent.