MiRNAs在骨关节炎发生发展中的调控作用

Regulatory role of microRNAs in the occurrence and development of osteoarthritis

背景:研究表明,miRNAs在炎症介导的疾病调控机制中具有重要意义,其参与细胞分化、凋亡、增殖、脂肪代谢、炎症、免疫应答等一系列的重要过程。目的:从骨关节炎发病机制,以及miRNAs与疼痛、衰老、早期诊断和治疗的关系等方面的研究进展对miRNAs在骨关节炎发生发展中的作用进行了综述,为骨关节炎的诊断及防治提供新思路。方法:检索知网数据库(CNKI)、维普数据库及万方数据平台、PubMed数据库、Biosos Preview数据库(BP)及Web of Science数据库(SCIE)1990至2020年的相关文献;英文检索词为“Osteoarthritis;miRNAs;Chondrocytes;Gene therapy”;中文检索词为“骨关节炎;miRNAs;软骨细胞;基因治疗”,对检索词进行组合后检索相关文献,排除重复性研究及年代久远的文献,最终纳入76篇符合标准的文献。结果与结论:①miRNAs是一类非编码蛋白质的小分子RNA,广泛存在于各种真核生物的细胞中。微小RNA广泛参与转录后基因水平的调控,由于与靶基因的结合而影响生物学过程及细胞信号传导,在骨关节炎的病程发展中起着非常重要的作用;②microRNA具有高度的时序性及组织特异性,通过调控miRNA在骨关节炎中表达水平的上调或下调,进而抑制骨关节炎发展。虽然microRNA在骨关节炎中的作用机制已有大量研究,但具体机制仍然不是非常明确。

BACKGROUND:Studies have shown that microRNAs play an important role in the regulation of inflammation-mediated diseases.miRNAs are involved in a series of processes such as cell differentiation,apoptosis,proliferation,fat metabolism,inflammation,and immune response.OBJECTIVE:To review the roles of microRNAs in the occurrence and development of osteoarthritis from the pathogenesis of osteoarthritis and the relationship between microRNAs and pain,aging,early diagnosis and treatment,so as to provide new ideas for the diagnosis,prevention and treatment of osteoarthritis.METHODS:CNKI,VIP,WanFang,PubMed,Biosos Preview and Web of Science were searched for relevant literatures published from 1990 to 2020.The search terms were“Osteoarthritis;miRNAs;Chondrocytes;Gene therapy”in Chinese and English,respectively.After excluding repetitive and obsolete literatures,76 literatures meeting the inclusion criteria were finally selected.RESULTS AND CONCLUSION:MicroRNA is a kind of non-coding protein small molecule RNA,which widely exists in various eukaryotic cells.MicroRNA is widely involved in the regulation of post-transcriptional genes,affects biological processes and cellular signal transduction due to its binding to target genes,and plays a very important role in the development of osteoarthritis.MicroRNAs participate in the regulation of post-transcriptional genes,affects cellular signal transduction and biological processes by binding to target genes,and plays an important regulatory role in the occurrence and development of osteoarthritis.Although the role of microRNAs in osteoarthritis has been studied a lot,the specific mechanism is still unclear.