circMTO1表达与miR-3200/PEBP1轴的关系及其预测内镜黏膜下剥离术后复发的价值

Relationship between the expression of circMTO1 and miR-3200/PEBP1 axis and its predictive effect on the recurrence after endoscopic submucosal dissection

目的研究circMTO1表达与miR-3200/磷脂酰乙醇胺结合蛋白1(PEBP1)轴的关系及其预测内镜黏膜下剥离术(ESD)后复发的价值。方法选择2015年1月-2018年7月在陆军军医大学第一附属医院(西南医院)接受ESD治疗的早期胃癌患者作为研究对象(早期胃癌组),另取同期经胃黏膜活检明确为正常胃黏膜的受试者作为对照组。检测两组circMTO1、miR-3200和PEBP1的表达水平,随访早期胃癌患者的无复发生存情况,并采用Kaplan-Meier曲线分析无复发生存率的差异。结果早期胃癌组circMTO1和PEBP1表达水平明显低于对照组,miR-3200表达水平明显高于对照组(P<0.05),且circMTO1与miR-3200呈负相关、与PEBP1呈正相关,miR-3200与PEBP1呈负相关;早期胃癌组中不同肿瘤直径、浸润深度、淋巴结转移情况和脉管癌栓情况的circMTO1、miR-3200和PEBP1表达水平比较,差异均有统计学意义(P<0.05);Kaplan-Meier曲线分析显示,circMTO1高表达的早期胃癌患者无复发生存率高于circMTO1低表达患者,miR-3200高表达的早期胃癌患者无复发生存率低于miR-3200低表达患者,PEBP1高表达的早期胃癌患者无复发生存率高于PEBP1低表达患者。结论早期胃癌中circMTO1低表达与病情恶化和ESD术后无复发生存时间缩短有关,靶向miR-3200/PEBP1轴可能是circMTO1参与早期胃癌发生发展的分子机制。

Objective To study the relationship between the expression of circMTO1,and miR-3200/PEBP1 axis and its predictive effect on the recurrence after endoscopic submucosal dissection(ESD).Methods Patients with early gastric cancer who received ESD treatment in the First Hospital Affiliated to AMU(Southwest Hospital)from January 2015 to July 2018 were selected as the research subjects(early gastric cancer group),and the patients who received endoscopic gastric mucosal biopsy and confirmed as normal gastric mucosa by pathology wereselected as the control group. The expression levels of circMTO1, miR-3200 and PEBP1 in early gastric cancer andnormal gastric mucosa were detected. The recurrence free survival of early gastric cancer patients was followed up.The difference of recurrence free survival rate was analyzed by Kaplan-Meier curve. Results The expression levelsof circMTO1 and PEBP1 in the early gastric cancer group were significantly lower than those in the control group, andthe expression levels of miR-3200 were significantly higher than that in the control group (P < 0.05), and circMTO1was negatively correlated with miR-3200 and positively correlated with PEBP1, and miR-3200 was negativelycorrelated with PEBP1;The expression levels of circMTO1, miR-3200 and PEBP1 were significantly different in theearly gastric cancer group with different tumor diameter, depth of invasion, lymph node metastasis and vascular tumorthrombus (P < 0.05);Kaplan-Meier curve analysis showed that the recurrence free survival rate of patients with highexpression of circMTO1 was higher than that of patients with low expression of circMTO1, the recurrence freesurvival rate of patients with high expression of miR-3200 was lower than that of patients with low expression of miR-3200, the recurrence free survival rate of patients with high expression of PEBP1 was higher than that of patients withlow expression of PEBP1. Conclusion The low expression of circMTO1 in early gastric cancer relates with thedeterioration and the shortening of recurrence free survival time after ESD, targeting miR-3200/PEBP1 axis may bethe molecular mechanism of circMTO1 participating in the occurrence and development of early gastric cancer.