CD3^(+)CD56^(+)NKT细胞及其细胞因子在Graves病患者体内的表达

Expression of CD3^(+)CD56^(+)NKT cells and their cytokines in patients with Graves disease

目的探讨CD3^(+)CD56^(+)NKT细胞(NKT细胞)及其细胞因子[白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)]在毒性弥漫性甲状腺肿[Graves病(GD)]患者体内的表达情况。方法选择19例GD患者作为实验组,另选择19例健康志愿者作为对照组。检测并比较两组甲状腺功能指标[血清游离三碘甲腺原氨酸(FT3)、血清游离甲状腺素(FT4)、促甲状腺激素(TSH)、甲状腺过氧化物酶抗体(TPOAb)]、IL-6、TNF-α及NKT细胞水平,IL-2、IL-10阳性率,探讨FT4、TSH、TPOAb与NKT细胞及细胞因子的相关性。结果实验组FT3、FT4、TPOAb、TNF-α和NKT细胞水平均高于对照组,TSH和IL-6水平均低于对照组,差异有统计学意义(P<0.05)。实验组IL-2和IL-10阳性率分别为89.47%、100.00%,均高于对照组的0、0,差异有统计学意义(P<0.05)。IL-2、IL-10、TNF-α、NKT细胞水平与FT4、TSH和TPOAb水平密切相关,IL-6水平与TSH水平密切相关(P<0.05)。以TSH水平为因变量,IL-2、IL-6、IL-10、TNF-α、NKT细胞水平为自变量,采用进入法纳入变量,结果显示IL-10水平与TSH水平呈负相关(P<0.05)。以TPOAb水平为因变量,IL-2、IL-6、IL-10、TNF-α和NKT细胞水平为自变量,采用进入法纳入变量,结果显示IL-10水平与TPOAb水平呈负相关(P<0.05)。结论GD患者外周血NKT细胞水平增加,其相关的细胞因子在GD发病中具有重要意义。

Objective To discuss the expression of CD3^(+)CD56^(+)NKT cells and their cytokines[interleukin-2(IL-2),interleukin-6(IL-6),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)]in patients with Graves disease(GD).Methods There were 19 GD patients selected as the experimental group,and 19 healthy volunteers as the control group.The thyroid function indexes[serum free triiodothyronine(FT3),serum free thyroxine(FT4),thyroid stimulating hormone(TSH),thyroid peroxidase antibody(TPOAb)],IL-6,TNF-α,NKT cell,positive IL-2 and IL-10 were detected and compared between the two groups,and correlation between FT4,TSH,TPOAb and NKT cells and cytokines was discussed.Results The levels of FT3,FT4,TPOAb,TNF-αand NKT cells of the experimental group were higher than those of the control group,and TSH and IL-6 were lower than those of the control group.All the difference was statistically significant(P<0.05).The positive rate of IL-2 and IL-10 of the experimental group were 89.47%and 100.00%,which were all higher than 0 and 0 of the control group,and the difference was statistically significant(P<0.05).IL-2,IL-10,TNF-α,NKT cell levels were closely related to FT4,TSH and TPOAb,and IL-6 were closely related to TSH(P<0.05).With TSH as the dependent variable,IL-2,IL-6,IL-10,TNF-α,NKT cell as independent variables,the entry method was used to include the variables,the results showed that IL-10 level and TSH were negatively correlated(P<0.05).With TPOAb level as the dependent variable,IL-2,IL-6,IL-10,TNF-αand NKT cell levels as independent variables,the entry method was used to include the variables,and the results showed that IL-10 was negatively correlated with TPOAb(P<0.05).Conclusion The level of NKT cells in the peripheral blood of GD patients is increased,and its related cytokines play an important role in the pathogenesis of GD.