耐受性树突状细胞通过降低Th1细胞和Th17细胞比例减轻CIA大鼠的关节炎症和病变

Tolerogenic dendritic cells alleviate joint inflammation and arthropathy via reducing Th1 and Th17 cell proportion in CIA rats

目的初步探讨核因子κB寡脱氧核苷酸诱骗剂(NF-κB ODN decoy)诱导建立的耐受性树突状细胞(tolDC)对胶原蛋白诱导性关节炎(CIA)大鼠1型辅助T(Th1)细胞、Th2细胞、Th17细胞、调节性T细胞(Treg)的影响及干预效果。方法取SD雌性大鼠建立CIA大鼠模型,设CIA模型组、牛Ⅱ型胶原蛋白-诱骗剂-树突状细胞(Col2-decoy DC)处理组、空白对照组、Col2-decoy DC对照组。于初次免疫的第20天尾静脉注射tolDC进行处理,造模第7周处死大鼠。流式细胞术检测大鼠脾脏Th1细胞、Th2细胞、Th17细胞、Treg比例,HE染色检测踝关节病变情况,并对关节炎指数(AI)进行评分。结果与CIA模型组相比,Col2-decoy DC组AI降低且踝关节病变减轻,脾脏CD4+T细胞中Th1细胞、Th17细胞百分率降低而Th2细胞、Treg百分率升高。结论tolDC通过降低CD4+T细胞中Th1细胞和Th17细胞比率减轻CIA大鼠炎症和关节病变。

Objective To investigate the effects of tolerogenic dendritic cells(tolDCs)induced by nuclear factorκB oligodeoxynucleotide decoy(NF-κB ODN decoy)on Th1 cells,Th2 cells,Th17 cells and regulatory T cells(Tregs)and the intervention effects on collagen-induced arthritis(CIA)rats.Methods SD female rats used to establish CIA rat models were divided into four groups,including a CIA model group,a bovine typeⅡcollagen-decoy-dendritic cell(Col2-decoy DC)treatment group,a blank control group,and a Col2-decoy DC control group.On the 20th days after the first immunization,the rats were injected with tolDCs via the tail vein,and the rats were sacrificed on the 7th weeks.The proportions of Th1 cells,Th2 cells,Th17 cells,and Tregs in the rat spleen were detected by flow cytometry.The ankle joint pathomorphological change was evaluated by HE staining,and the arthritis index(AI)was scored.Results Compared with the CIA model group,the Col2-decoy DC group had lower AI and milder ankle joint pathomorphological change.The percentages of Th1 cells and Th17 cells in the spleen CD4+T cells decreased,while the percentages of Th2 cells and Tregs increased.Conclusion The treatment of tolDCs can alleviate the inflammation and arthropathy of CIA rats by reducing the proportion of Th1 and Th17 cells in CD4+T cells.