基于色泽量化与主要成分含量的不同基原莪术饮片质量识别研究

Study on quality identification of Curcumae Rhizoma from different origins based on quantitative analysis of appearance color and content of main components

利用分光测色仪测定不同基原莪术饮片粉末L^(*),a^(*),b^(*),通过SPSS 21.0进行判别分析建立不同基原莪术饮片颜色范围及数学预测模型,广西莪术L^(*),a^(*),b^(*)分别在58.09~62.40,4.53~5.66,23.61~24.29,蓬莪术L^(*),a^(*),b^(*)分别在64.02~70.71,-0.89~4.13,44.59~54.52,温莪术L^(*),a^(*),b^(*)分别在68.55~70.99,-0.11~1.47,28.26~32.19;所建立的数学预测模型通过原始和交叉验证及外部样品验证表明可实现不同基原莪术100%识别。建立一种双波长HPLC同时测定9种倍半萜类化合物及3种姜黄素类成分含量的方法并测定不同基原莪术的含量,结果表明广西莪术中新莪术二酮、异莪术烯醇、β-榄香烯含量较高,蓬莪术中莪术烯醇、呋喃二烯酮、去甲氧基姜黄素、姜黄素含量较高,温莪术以莪术二酮、呋喃二烯、吉马酮3种成分为主。对L^(*),a^(*),b^(*)与12种成分含量进行Pearson相关性分析,结果表明莪术烯醇、呋喃二烯酮、去甲氧基姜黄素、姜黄素与b^(*)(蓝黄轴)有显著正相关(P<0.01),新莪术二酮、β-榄香烯、异莪术烯醇与L^(*)(亮度)呈显著负相关(P<0.01),莪术二酮、呋喃二烯、吉马酮与L^(*)有显著正相关(P<0.01),表明莪术的外观颜色可反映内在成分含量的变化。该研究为不同基原莪术饮片的快速识别及质量评价体系的建立提供参考。

L^(*),a^(*)and b^(*)values of prepared slices of Curcumae Rhizoma were measured by spectrophotometer.SPSS 21.0 was used for discriminant analysis to establish the color range and mathematical prediction model of prepared slices of Curcumae Rhizoma.The values of L^(*),a^(*)and b^(*)of kwangsiensis ranged from 58.09-62.40,4.53-5.66 and 23.61-24.29,while the values of L^(*),a^(*)and b^(*)of phaeocaulis were between 64.02-70.71,-0.89-4.13 and 44.59-54.52,respectively.The values of L^(*),a^(*)and b^(*)of wenyujin were 68.55-70.99,-0.11-1.47 and 28.26-32.19,respectively.The mathematical prediction model was proved to be able to realize 100%identification of Curcumae Rhizome of different origins through original and cross validation and external samples validation.A dual wavelength HPLC was established;the contents of 9 sesquiterpenoids and 3 Curcumae Rhizomes were determined simultaneously;and the contents of Curcumae Rhizome of different origins were determined.The results showed that kwangsiensis had higher contents of neocurdione,β-elemene and isocurcumaenol,phaeocaulis curcumin,furadienone,demethoxycurcumin and curcumin;and wenyujin mainly contained curdione,furadienes and guimarone.Pearson correlation analysis on L^(*),a^(*),b^(*)value and content of 12 components showed that curcumin,furadienone,demethoxycurcumin and curcumin had a significant positive correlation with b^(*)value(P<0.01).There was a significant negative correlation between neocurdione,β-elemene and isocurcumaenol and L^(*)value(P<0.01).Curdione,furadienes and guimarone were significantly correlated with L^(*)value(P<0.01),indicating that the appearance co-lor of Curcumae Rhizoma could reflect the change of the content of the internal components.This study provided reference for the rapid recognition of Curcumae Rhizoma and the establishment of quality evaluation system.