基于UPLC-Q-Exactive Orbitrap-MS分析甘草地上部分主要化学成分

Main chemical constituents in aerial parts of Glycyrrhiza uralensis by UPLC-Q-Exactive Orbitrap-MS

基于UPLC-Q-Exactive Orbitrap-MS技术对甘草地上部分的主要化学成分进行定性分析。采用Waters Acquity UPLC BEH-C18色谱柱(2.1 mm×100 mm, 1.7μm),以乙腈(A)-0.5%甲酸水溶液(B)为流动相梯度洗脱,流速0.2 mL·min^(-1),在电喷雾正负离子模式下采集数据。依据高分辨质谱提供的准分子离子峰和碎片离子的精确分子质量信息,确证了有关物质及其特征碎片离子的分子组成。再结合对照品对照与相关文献数据支持,最终鉴定出甘草地上部分的55种化学成分,包括42个黄酮类,9个茋类,2个香豆素类,1个木脂素类和1个酚酸类化合物。该方法可对甘草地上部分的化学成分进行快速、准确的定性分析,为今后进一步阐明其药效物质基础和资源开发利用提供科学依据。

Chemical constituents from aerial parts of Glycyrrhiza uralensis were analyzed and identified using ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS). The chromatographic column of Waters Acquity UPLC BEH-C18(2.1 mm×100 mm, 1.7 μm) was adopted, with acetonitrile-water(0.5% formic acid) as mobile phase at a flow rate of 0.2 mL·min^(-1). Data was collected in positive and negative modes of electrospray ionization(ESI). A total of 55 compounds, including 42 flavonoids, 9 stilbenes, 2 coumarins, 1 lignin and 1 phenolic acid, which were characterized in the aerial parts of G. uralensis based on accurate molecular mass information of molecular and product ions provided by UPLC-Q-Exactive Orbitrap-MS based on comparison with standard substances and references. It is an effective and accurate method to provide chemical information of constituents in aerial parts of G. uralensis, and can provide a reference for further study on pharmacodynamic material basis and resources development and utilization.