检测胃蛋白酶原I的便携式荧光测试系统

Portable Fluorescence Detection System for Measuring Pepsinogen I

人体血清中的胃蛋白酶原I是临床医学中诊断胃食管反流的常用生物学指标,而传统的检测方法存在测试时间长、成本高、灵敏度低等缺点。设计了一种能够快速定量检测胃蛋白酶原I的荧光测试系统。首先使用便携式显微镜采集样品的荧光图像,再利用RGB颜色通道中R通道图像处理算法对荧光图像进行检测和分析。最后根据实验数据,拟合出标准曲线,拟合度为99.556%,且检测结果的变异系数小于4.9%。结果表明,所设计的检测系统能够准确、快速地定量检测胃蛋白酶原I的浓度。

Pepsinogen I in human serum is a common biomarker for diagnosing gastroesophageal reflux in clinical medicine.Traditional detection methods for pepsinogen I have the disadvantages of being timeconsuming and having high cost and low sensitivity.In this study,a fluorescence detection system for the rapid and quantitative measurement of pepsinogen I is designed.First,a portable microscope is used to take a fluorescence image of the samples,and then the R channel imageprocessing algorithm of the RGB color mode is used to detect and analyze the images.Finally,according to experimental data,a standard curve is fitted with a fitting degree of 99.556%,and the variation coefficient of the test results is<4.9%.The experimental results show that the designed detection system can accurately and rapidly quantify pepsinogen I concentration.