PTEN诱导激酶1在预测胃癌患者预后及化疗疗效中的作用

Role of PINK1 in predicting prognosis and chemotherapy efficacy in patients with gastric cancer

目的探讨PTEN诱导激酶1(PTEN⁃induced kinas 1,PINK1)在预测胃癌患者预后及化疗疗效中的作用。方法利用KM⁃Plotter网站数据进行PINK1表达与胃癌患者预后及化疗疗效的相关性分析;收集3对新鲜胃癌及癌旁标本和153例胃癌石蜡标本及相应临床数据进行PINK1蛋白表达检测及生存分析验证,并对PINK1表达及其预测胃癌患者预后及化疗疗效中的作用进一步分析及验证。结果KM⁃Plotter网站数据分析显示,PINK1在胃癌组织中显著高表达,且无论分期及是否出现远处转移,其高表达均提示不良预后;PINK1高表达与5⁃Fu辅助化疗疗效良好相关。在153例胃癌石蜡标本及3对新鲜胃癌及癌旁标本中验证发现PINK1在胃癌组织中显著高表达,且其表达随分期的升高而增加;在发生远处转移或淋巴结转移的胃癌患者中表达量更高。预测胃癌患者预后不良时,PINK1免疫组化评分截断值4.50,AUC为0.772,敏感性为69.2%,特异性为76.0%;预测胃癌患者5⁃Fu辅助化疗疗效良好时,PINK1免疫组化评分截断值5.50,AUC为0.735,敏感性为60.0%,特异性为76.9%。结论PINK1在胃癌中高表达并在预测胃癌患者预后及化疗疗效中起一定作用。

Objective To explore the role of PTEN⁃induced kinas 1(PINK1)in predicting prognosis and chemotherapy efficacy in patients with gastric cancer(GC).Methods A correlation analysis on association of PINK1 expression with prognosis and chemotherapy efficacy was made by using the data from the KM⁃Plotter website.Three pairs of fresh GC and precancerous tissue samples,153 samples of formalin⁃embedded GC,and clinical data were collected to detect protein expression of PINK1,and further analyze role of PINK1 in predicting prognosis and chemotherapy efficacy in GC patients.Results KM⁃Plotter data analysis showed that PINK1 was highly expressed in patients with GC and indicated poor prognosis regardless of tumor staging and presence or absence of distant metastasis.High expression of PINK1 indicated favorable 5⁃Fu chemotherapy efficacy.In 153 samples of formalin⁃embedded GC tissue and 6 pairs of fresh GC tissue and normal adjacent tissue samples,PINK1 were highly expressed and their expressions were increasing with a rise in tumor staging,especially in those with distant metastasis or lymph node metastasis.In prediction of poor prognosis,cut⁃off value of PINK1 expression was 4.50,Area Under the Curve(AUC)was 0.772,sensitivity was 69.2%,and specificity was 76.0%;in prediction of 5⁃Fu chemotherapy efficacy,cut⁃off value of PINK1 expression was 5.50,AUC was 0.735,sensitivity was 60.0%,and specificity was 76.9%.Conclusions PINK1 is highly expressed in GC and plays a role in predicting prognosis and chemotherapy efficacy in patients with GC.