遵义市1岁以下维生素A缺乏留守儿童尿液氢核磁共振代谢组学研究

Study on the metabonomics of vitamin A deficiency urine of left-behind children under 1 year old in Zunyi area based on hydrogen-nuclear magnetic resonance

目的探讨氢核磁共振(1H-NMR)技术在维生素A缺乏(VAD)早期筛查中的应用价值。方法2019年1月至6月在遵义地区收集40例确诊VAD<1岁留守儿童为VAD组,另选取40例健康儿童为健康对照组,采集2组儿童尿液,测定1H-NMR波谱。应用代谢组学技术,结合多变量统计分析比较2组代谢产物,筛选出VAD患儿差异性代谢产物。结果健康对照组血清视黄醇水平[(1.35±0.18)μmol/L]与VAD组[(0.56±0.10)μmol/L]比较,差异有统计学意义(t=23.93,P<0.001)。尿液1H-NMR光谱图结果显示:与健康对照组比较,VAD组儿童尿液中甲基丙二酸、2-酮异戊酸酯、N-乙酰基糖蛋白、二甲基甘氨酸、三甲铵乙内酯水平明显升高,差异均有统计学意义(|r|>0.602,均P<0.05);异戊基丙基甘氨酸、2-羟基异丁酸酯、肌酸、磷酸胆碱/甘油磷酸胆碱、马尿酸盐水平明显下降,差异均有统计学意义(|r|>0.602,均P<0.05)。结论VAD患儿与健康儿童比较,尿液代谢产物存在差异,差异代谢产物与肠道微生态平衡、消化系统疾病、呼吸系统疾病、免疫相关疾病及能量代谢、生长发育密切相关,尿液1H-NMR代谢组学分析在VAD早期筛查中具有潜在应用价值。

Objective Explore the hydrogen-nuclear magnetic resonance(1H-NMR)application potential of metabolomics in early screening of vitamin A deficiency(VAD).Methods From January to June 2019,40 cases of left-behind children with confirmed VAD<1 year old were enrolled in Zunyi area as VAD group,and 40 healthy children were selected as healthy control group.Urine from the 2 groups of children was collected and 1H-NMR spectrum was measured.By combining metabonomics technology and multivariate statistical analysis,the 2 groups of metabolites were compared,and the differential metabolites of children with VAD were screened out.Results Compared with VAD group[(0.56±0.10)μmol/L],the difference in the serum retinol concentration of healthy control group[(1.35±0.18)μmol/L]was statistically significant(t=23.93,P<0.001).Compared with healthy group,VAD group showed higher level of methylmalonate,2-ketoisovalerat,N-acetyl glycoprotein signal,dimethylglycine and betaine,with statistically significant differences(|r|>0.602,all P<0.05).The level of Isovalerylglycine,2-hydroxyisobutyrate,creatine,phosphate choline/glycerophosphocholine,4-PY and Hippurate were significantly decreased,with statistically significant differences(|r|>0.602,all P<0.05).Conclusions Compared with healthy children,children with VAD have differences in urine Metabolites.Differential metabolites are closely correlated with intestinal microecological ba-lance,digestive system diseases,respiratory system diseases,immune-related diseases,energy metabolism,growth and development.Urine 1H-NMR metabolomics analysis has potential application value in the early screening of VAD.