PI3K/AKT信号通路抑制剂对食管癌细胞TE1的侵袭及迁移影响的机制研究

Mechanisms of PI3KAKT Signaling Pathway Inhibitors on Invasion and Migration of Esophageal Cancer Cell TE1

目的:探讨PI3K/AKT信号通路抑制剂对食管癌细胞TE1迁移及侵袭能力的影响,并分析其可能机制。方法:培养食管癌TE1细胞株,用30 μmol·L^(-1)的LY294002处理TE1细胞1 h,24 h后采用四甲基偶氮唑蓝(MTT)法测定细胞增殖抑制率,采用细胞划痕实验、细胞侵袭实验观察细胞迁移及侵袭情况,采用Western blot实验检测PY14 Cav–1的表达水平。结果:LY294002能明显抑制食管癌细胞株TE1的增殖,并抑制其迁移及侵袭能力,与对照组比较,差异具有统计学意义(P<0.05)。同时LY294002能够使PY14Cav–1表达水平下调。结论:PI3K/AKT信号通路正向调节食管癌细胞的增殖,并参与细胞的侵袭及迁移,LY294002能够抑制食管癌细胞的生长、迁移及侵袭,其可能机制与通过下调PY14 Cav–1表达水平有关。

Objective To investigate the effects of PI3K/AKT signaling pathway inhibitor on the invasion and migration of esophageal cancer cell TE1,and to discusse the possible mechanism.Methods TE1 cells were cultured and treated with LY294002 (30 μmol/L) for 1 hour.The inhibition rate of cell proliferation was measured by MTT 24 hours later.Cell migration and invasion were observed by cell scratch test and cell invasion test.The expression level of PY14 Cav–1 was detected by Western blot.Results LY294002 could significantly inhibit the growth of TE1 cell and inhibit its migration and invasion ability.There was a significant difference between LY294002 group and control group (P < 0.05).At the same time,LY294002 could down-regulate the expression of PY14 Cav–1.Conclusion PI3K/AKT signaling pathway positively regulates the proliferation of esophageal cancer cells and participates in cells invasion and migration.LY294002 can inhibit the growth,migration and invasion of esophageal cancer cells.Its possible mechanism is related to down-regulation of PY14Cav–1 expression.