26种圈养珍稀鸟类性别的快速分子生物学鉴定

Rapid molecular identification of the sex of 26 species of rare birds in captivity

世界上过半数的鸟类为单态性,雌雄难以分辨,对人工饲养繁育造成困难,快速准确地性别鉴定对鸟类繁育研究尤为重要。本文根据已有研究,采用无侵入采样的方式,应用3对引物(2550F/2718R、P2/P8和K7/W1)分别对26种258只鸟类的羽毛样品进行了性别特异性PCR扩增,其中部分产物进一步酶切,经琼脂糖凝胶电泳。结果发现:白鹈鹕雄性为1条321 bp条带,雌性多1条234 bp条带;斑嘴环企鹅雄性为302 bp和50~100 bp条带,雌性多1条367 bp条带;鹤鸵雄性样品为1条约350 bp条带,雌性多1条约150 bp条带。结果表明,羽根作为样品,未经DNA提取直接进行PCR检测,可有效地进行鸟类性别鉴定。

Over half of the birds in the world are monomorphic, which makes it difficult to distinguish between the males and females in captive breeding and studies. It is vital to determine the sex of these birds rapidly and accurately. In this paper, based on previous studies, gender-specific PCR and restriction endonuclease reactions were performed on the feather root samples of 258 birds of 26 avian species. Agarose gel electrophoresis showed that male Rosy Pelicans amplified a 321 bp band, but the females had an extra 234 bp band;that male African Penguins amplified a 302 bp band and a 50-100 bp band, but the females did with an additional 367 bp band;and that male Cassowaries showed a 350 bp band, but the females had an additional 150 bp band. Feather roots as samples are sufficient to be effectively used for sex identification for rare birds using PCR detection, without DNA extraction.