摘要
制备了壳聚糖/β-甘油磷酸钠(CS/β-GP)复合物水凝胶,测试其温敏特性及其SEM、FTIR性质,并研究人卵巢癌细胞、人宫颈癌细胞和小鼠成纤维细胞在包裹CS/β-GP水凝胶后的运动迁移性能和生理活性,进一步探究温敏性水凝胶在肿瘤治疗领域的潜在应用。方法制备CS/β-GP水凝胶,并将该水凝胶包裹于生长良好的人卵巢癌细胞、人宫颈癌细胞和小鼠成纤维细胞表面继续培养,通过细胞划痕实验和活死荧光染色检测水凝胶对癌细胞的迁移性和生理活性的影响。结果制备出的CS/β-GP水凝胶在室温下呈透明液体,在37℃发生凝胶化现象;细胞划痕实验结果显示,未涂覆水凝胶的癌细胞随培养时间的延长细胞不断向划痕区迁移生长,而涂覆了水凝胶之后,细胞迁移明显受到抑制;活死荧光染色结果表明,水凝胶可抑制癌细胞(人卵巢癌细胞、人宫颈癌细胞)生理活性,但对正常细胞(小鼠成纤维细胞)的生理活性影响较小,故此CS/β-GP水凝胶的温敏特性可以作为癌细胞与正常细胞的初筛,有望用于生物安全的癌症治疗。
The chitosan/β-glycerol phosphate sodium(CS/β-GP)complex hydrogel is prepared,and its temperature-sensitive properties,SEM and FTIR are tested.In addition,the migration properties and physiological activities of human ovarian cancer cells,human cervical cancer cells and mouse fibroblasts encapsulated with CS/β-GP hydrogel are studied.We further explore the potential applications of temperature-sensitive hydrogels in the field of tumor therapy.METHOD A hydrogel based on CS/β-GP complex is prepared and coated on the surface of well-growth human ovarian cancer cells.The effect of hydrogel on the migration and physiological activity of cancer cells is examined by cell scratch assay and live/dead fluorescent staining.RESULTS The prepared CS/β-GP hydrogel is a clear liquid at room temperature and gelled at 37℃.The results of cell scratch test show that the uncoated hydrogel cells gradually migrated to the scratched area with the prolongation of the culture time,and the cell migration growth is obviously inhibited after the hydrogel is coated.The results of live/dead fluorescent staining show that the hydrogel can cause the cancer cells to lose physiological activity,but has little effect on the physiological activity of normal cells.Therefore,the temperature-sensitive properties of CS/β-GP hydrogels are expected to be used for cancer treatment without affecting normal cells.
作者
许浩
魏延
徐双梦
苏慧
李涵琴
黄棣
王楷群
胡银春
XU Hao;WEI Yan;XU Shuangmeng;SU Hui;LI Hanqin;HUANG Di;WANG Kaiqun;HU Yinchun(Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering,Department of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China;Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering,Taiyuan University of Technology, Taiyuan 030024, China)
出处
《功能材料》
EI
CAS
CSCD
北大核心
2021年第5期5121-5126,共6页
Journal of Functional Materials
基金
国家自然科学基金青年资助项目(51503140,11502158,11802197)
山西省应用基础研究计划面上资助项目(201901D111077)
山西省回国留学人员科研资助项目(HGKY2019037)。
关键词
水凝胶
温敏性
壳化
癌症治疗
壳聚糖
hydrogel
thermo sensitivity
shellization
cancer treatmen
chitosan