定量蛋白质组学常用研究技术及其在肝源性疾病发病机制中的应用进展

Commonly used quantitative proteomics research techniques and their application in the study of pathogenesis of liver-derived diseases

蛋白质组学是以蛋白质组为研究对象的学科,是后基因组时代重要的组学技术。蛋白质是基因表达的最终产物,蛋白质表达的整体研究,对疾病机制、药物疗效研究意义重大,其中的定量蛋白质组学近年来应用广泛。肝脏是体内最重要的药物代谢器官,超过70%的药物经过肝脏代谢。本文旨在介绍定量蛋白质组学目前为止的常用技术,以及近五年定量蛋白质组学在药物性肝损伤、非酒精性脂肪性肝病、肝纤维化和肝硬化、肝细胞癌等常见肝病中的应用进展。

Proteomics is a subject with proteome as its research object.It is an important omics technology in the post-genomic era.Protein is the final product of gene expression.The overall study of protein expression is of great significance to the study of disease mechanisms and drug efficacy.Among them,quantitative proteomics has been widely used in recent years.The liver is the most important drug metabolism organ in the body,and more than 70%of drugs are metabolized by the liver.This article introduces the commonly used proteomics technology so far,as well as the progress of quantitative proteomics in drug-induced liver injury,non-alcoholic fatty liver disease,liver fibrosis and cirrhosis,hepatocellular carcinoma in the past five years.